Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4038..4630 Replicon plasmid unnamed1
Accession NZ_CP040594
Organism Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag FFY44_RS25945 Protein ID WP_000520549.1
Coordinates 4382..4630 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag FFY44_RS25940 Protein ID WP_000681613.1
Coordinates 4038..4385 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFY44_RS25905 41..313 - 273 WP_001328551.1 TrbC/VirB2 family protein -
FFY44_RS25910 381..1001 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
FFY44_RS25915 1359..1574 - 216 WP_000760375.1 EexN family lipoprotein -
FFY44_RS25920 1571..1864 - 294 WP_000134733.1 LuxR family transcriptional regulator -
FFY44_RS25925 1941..3170 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
FFY44_RS25930 3174..3677 - 504 WP_000781813.1 hypothetical protein -
FFY44_RS25940 4038..4385 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FFY44_RS25945 4382..4630 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
FFY44_RS25950 4935..5285 - 351 WP_000854259.1 hypothetical protein -
FFY44_RS25955 5376..5675 - 300 WP_000650309.1 hypothetical protein -
FFY44_RS25965 6127..6354 - 228 WP_000801440.1 hypothetical protein -
FFY44_RS25970 6341..6592 - 252 WP_001230707.1 hypothetical protein -
FFY44_RS25975 6589..7131 - 543 WP_001083906.1 hypothetical protein -
FFY44_RS25980 7236..7751 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31184 31184


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T126128 WP_000520549.1 NZ_CP040594:c4630-4382 [Klebsiella pneumoniae subsp. pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T126128 NZ_CP040594:c4630-4382 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT126128 WP_000681613.1 NZ_CP040594:c4385-4038 [Klebsiella pneumoniae subsp. pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT126128 NZ_CP040594:c4385-4038 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References