Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 67125..67650 Replicon plasmid pCFSAN059540
Accession NZ_CP040573
Organism Escherichia coli O157:H7 strain ECP17-46

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag FGA21_RS28125 Protein ID WP_001159868.1
Coordinates 67345..67650 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FGA21_RS28120 Protein ID WP_000813634.1
Coordinates 67125..67343 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGA21_RS28085 62408..62638 + 231 WP_010891288.1 hypothetical protein -
FGA21_RS28090 62759..63499 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
FGA21_RS28095 63784..64761 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
FGA21_RS28105 65169..65369 - 201 WP_000708307.1 hypothetical protein -
FGA21_RS28110 65366..65986 - 621 WP_001248529.1 hypothetical protein -
FGA21_RS28115 65983..66666 - 684 WP_010891291.1 DNA-binding protein -
FGA21_RS28120 67125..67343 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FGA21_RS28125 67345..67650 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
FGA21_RS28130 67651..68457 + 807 WP_000016989.1 site-specific integrase -
FGA21_RS28145 70447..70809 + 363 Protein_66 RepB family plasmid replication initiator protein -
FGA21_RS28150 71397..72563 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD 1..94177 94177


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T126105 WP_001159868.1 NZ_CP040573:67345-67650 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T126105 NZ_CP040573:67345-67650 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT126105 WP_000813634.1 NZ_CP040573:67125-67343 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT126105 NZ_CP040573:67125-67343 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References