Detailed information of TA system TA126083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3042444..3042915
• Replicon: chromosome
• Accession: NZ_CP040572
• Organism: Escherichia coli O157:H7 strain ECP17-46

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: FGA21_RS15985
• Protein ID: WP_001303511.1
• Coordinates: 3042637..3042915 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: FGA21_RS15980
• Protein ID: WP_001302048.1
• Coordinates: 3042444..3042635 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGA21_RS15940	3037651..3038235	-	585	WP_000206793.1	DUF551 domain-containing protein	-
FGA21_RS15945	3038292..3038687	-	396	WP_001118159.1	DUF977 family protein	-
FGA21_RS15950	3038703..3039472	-	770	Protein_3036	DUF1627 domain-containing protein	-
FGA21_RS15955	3039498..3040238	-	741	WP_000788938.1	ATP-binding protein	-
FGA21_RS15960	3040245..3041207	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
FGA21_RS15965	3041230..3041655	-	426	WP_000693943.1	Rha family transcriptional regulator	-
FGA21_RS15970	3041652..3041954	-	303	WP_000172738.1	transcriptional regulator	-
FGA21_RS15975	3042052..3042423	+	372	WP_001169686.1	hypothetical protein	-
FGA21_RS15980	3042444..3042635	+	192	WP_001302048.1	hypothetical protein	Antitoxin
FGA21_RS15985	3042637..3042915	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGA21_RS15990	3043267..3043566	+	300	WP_001240334.1	hypothetical protein	-
FGA21_RS15995	3043638..3043856	+	219	WP_001171930.1	hypothetical protein	-
FGA21_RS16005	3044425..3044613	+	189	WP_000449192.1	cell division inhibition protein DicB	-
FGA21_RS16010	3044610..3044801	+	192	WP_001090200.1	DUF1482 family protein	-
FGA21_RS16015	3044894..3047365	+	2472	WP_000048458.1	exonuclease	-
FGA21_RS16020	3047438..3047689	+	252	WP_000005552.1	excisionase family protein	-
FGA21_RS16025	3047724..3047911	+	188	Protein_3050	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2953041..3051201	98160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T126083 WP_001303511.1 NZ_CP040572:3042637-3042915 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T126083 NZ_CP040572:3042637-3042915 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT126083 WP_001302048.1 NZ_CP040572:3042444-3042635 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT126083 NZ_CP040572:3042444-3042635 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0