Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 325843..326474 Replicon chromosome
Accession NZ_CP040572
Organism Escherichia coli O157:H7 strain ECP17-46

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag FGA21_RS01560 Protein ID WP_001259385.1
Coordinates 325843..326118 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FGA21_RS01565 Protein ID WP_000593555.1
Coordinates 326115..326474 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGA21_RS01545 320847..321914 + 1068 WP_000361458.1 HlyD family secretion protein -
FGA21_RS01550 321911..324646 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
FGA21_RS01555 324646..325770 + 1125 WP_001301659.1 ABC transporter permease -
FGA21_RS01560 325843..326118 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
FGA21_RS01565 326115..326474 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FGA21_RS01570 326524..327384 - 861 WP_001131788.1 class II aldolase -
FGA21_RS01575 327416..327685 - 270 WP_000933060.1 HPr family phosphocarrier protein -
FGA21_RS01580 327675..329183 - 1509 WP_001301773.1 carbohydrate kinase -
FGA21_RS01585 329176..330534 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
FGA21_RS01590 330611..330892 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
FGA21_RS01595 330889..331362 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 316987..326474 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T126069 WP_001259385.1 NZ_CP040572:325843-326118 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T126069 NZ_CP040572:325843-326118 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT126069 WP_000593555.1 NZ_CP040572:326115-326474 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT126069 NZ_CP040572:326115-326474 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References