Detailed information of TA system TA126066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 17594..18153
• Replicon: plasmid pCFSAN059541
• Accession: NZ_CP040571
• Organism: Escherichia coli O157:H7 strain ECP17-1298

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: FGA23_RS27805
• Protein ID: WP_000421248.1
• Coordinates: 17878..18153 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: FGA23_RS27800
• Protein ID: WP_001178089.1
• Coordinates: 17594..17878 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGA23_RS27745	12785..13345	+	561	WP_000840472.1	fertility inhibition protein FinO	-
FGA23_RS27750	13478..13690	+	213	WP_001302189.1	ANR family transcriptional regulator	-
FGA23_RS27755	13935..14396	+	462	WP_001233853.1	thermonuclease family protein	-
FGA23_RS27760	14442..14651	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
FGA23_RS27765	14689..15027	+	339	WP_000766796.1	hypothetical protein	-
FGA23_RS27770	15267..15521	+	255	WP_000083831.1	replication regulatory protein RepA	-
FGA23_RS27780	15757..15831	+	75	WP_001370046.1	RepA leader peptide Tap	-
FGA23_RS27785	15824..16681	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
FGA23_RS27800	17594..17878	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
FGA23_RS27805	17878..18153	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGA23_RS27810	18248..18454	+	207	WP_001105064.1	hypothetical protein	-
FGA23_RS27815	18554..18769	-	216	Protein_18	transposase	-
FGA23_RS27820	18813..19379	-	567	Protein_19	IS3-like element IS600 family transposase	-
FGA23_RS27825	19379..19795	-	417	Protein_20	IS91 family transposase	-
FGA23_RS27830	19795..19980	-	186	WP_001302179.1	hypothetical protein	-
FGA23_RS27835	20157..22367	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
FGA23_RS27840	22411..22800	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD	1..92763	92763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T126066 WP_000421248.1 NZ_CP040571:17878-18153 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T126066 NZ_CP040571:17878-18153 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT126066 WP_001178089.1 NZ_CP040571:17594-17878 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT126066 NZ_CP040571:17594-17878 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8