Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4121181..4121799 Replicon chromosome
Accession NZ_CP040570
Organism Escherichia coli O157:H7 strain ECP17-1298

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FGA23_RS21520 Protein ID WP_001291435.1
Coordinates 4121581..4121799 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FGA23_RS21515 Protein ID WP_000344800.1
Coordinates 4121181..4121555 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGA23_RS21505 4116270..4117463 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FGA23_RS21510 4117486..4120635 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
FGA23_RS21515 4121181..4121555 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FGA23_RS21520 4121581..4121799 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FGA23_RS21525 4121971..4122522 + 552 WP_000102553.1 maltose O-acetyltransferase -
FGA23_RS21530 4122638..4123108 + 471 WP_000136192.1 YlaC family protein -
FGA23_RS21535 4123272..4124822 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FGA23_RS21540 4124864..4125217 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FGA23_RS21550 4125596..4125907 + 312 WP_000409911.1 MGMT family protein -
FGA23_RS21555 4125940..4126512 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T126058 WP_001291435.1 NZ_CP040570:4121581-4121799 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T126058 NZ_CP040570:4121581-4121799 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT126058 WP_000344800.1 NZ_CP040570:4121181-4121555 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT126058 NZ_CP040570:4121181-4121555 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References