Detailed information of TA system TA126021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2135526..2136055
• Replicon: chromosome
• Accession: NZ_CP040560
• Organism: Staphylococcus aureus strain Col52-A5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FA001_RS10925
• Protein ID: WP_000621175.1
• Coordinates: 2135526..2135888 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FA001_RS10930
• Protein ID: WP_000948331.1
• Coordinates: 2135885..2136055 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FA001_RS10905	2132504..2133274	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
FA001_RS10910	2133249..2133728	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
FA001_RS10915	2133730..2134056	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FA001_RS10920	2134175..2135176	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FA001_RS10925	2135526..2135888	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FA001_RS10930	2135885..2136055	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FA001_RS10935	2136140..2137288	-	1149	WP_001281145.1	alanine racemase	-
FA001_RS10940	2137354..2137713	-	360	WP_000581200.1	holo-ACP synthase	-
FA001_RS10945	2137717..2138208	-	492	WP_001205910.1	PH domain-containing protein	-
FA001_RS10950	2138195..2139778	-	1584	WP_001294626.1	PH domain-containing protein	-
FA001_RS10955	2139771..2140250	-	480	WP_001287088.1	hypothetical protein	-
FA001_RS10960	2140458..2141018	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T126021 WP_000621175.1 NZ_CP040560:c2135888-2135526 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T126021 NZ_CP040560:c2135888-2135526 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT126021 WP_000948331.1 NZ_CP040560:c2136055-2135885 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT126021 NZ_CP040560:c2136055-2135885 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1