Detailed information of TA system TA125999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 121500..122170
• Replicon: plasmid pCR-HvKP5-VIR
• Accession: NZ_CP040546
• Organism: Klebsiella pneumoniae strain CR-HvKP5

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: FFT49_RS27545
• Protein ID: WP_004213072.1
• Coordinates: 121500..121943 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: FFT49_RS27550
• Protein ID: WP_004213073.1
• Coordinates: 121940..122170 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT49_RS27495	116909..117184	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
FFT49_RS27500	117247..117738	+	492	WP_011251327.1	DM13 domain-containing protein	-
FFT49_RS27505	117787..118707	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
FFT49_RS27515	118798..119204	+	407	Protein_127	GAF domain-containing protein	-
FFT49_RS27535	120773..121066	+	294	Protein_128	transposase	-
FFT49_RS27540	121070..121486	+	417	Protein_129	restriction endonuclease	-
FFT49_RS27545	121500..121943	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFT49_RS27550	121940..122170	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FFT49_RS27555	122778..123911	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
FFT49_RS27560	123927..124220	+	294	WP_004213076.1	hypothetical protein	-
FFT49_RS27565	124210..124416	-	207	WP_004213077.1	hypothetical protein	-
FFT49_RS27570	124768..125058	+	291	WP_004213078.1	hypothetical protein	-
FFT49_RS27575	125048..125947	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2	1..177694	177694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T125999 WP_004213072.1 NZ_CP040546:c121943-121500 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T125999 NZ_CP040546:c121943-121500 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT125999 WP_004213073.1 NZ_CP040546:c122170-121940 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT125999 NZ_CP040546:c122170-121940 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620