Detailed information of TA system TA125979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2200..2674
• Replicon: plasmid pCR-HvKP4-p5
• Accession: NZ_CP040544
• Organism: Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: FFT48_RS29930
• Protein ID: WP_004197762.1
• Coordinates: 2408..2674 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: FFT48_RS29925
• Protein ID: WP_000879771.1
• Coordinates: 2200..2424 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT48_RS29910	285..830	+	546	Protein_1	relaxase/mobilization nuclease domain-containing protein	-
FFT48_RS29915	805..1233	+	429	Protein_2	MbeB family mobilization protein	-
FFT48_RS29920	1321..1533	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
FFT48_RS29925	2200..2424	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
FFT48_RS29930	2408..2674	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFT48_RS29935	2744..3094	-	351	WP_004197756.1	hypothetical protein	-
FFT48_RS29940	3160..3705	-	546	WP_023317533.1	hypothetical protein	-
FFT48_RS29945	3731..4110	-	380	Protein_8	hypothetical protein	-
FFT48_RS30635	5213..5389	+	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T125979 WP_004197762.1 NZ_CP040544:2408-2674 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T125979 NZ_CP040544:2408-2674 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT125979 WP_000879771.1 NZ_CP040544:2200-2424 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT125979 NZ_CP040544:2200-2424 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5