Detailed information of TA system TA125978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 7810..8390
• Replicon: plasmid pCR-HvKP4-p4
• Accession: NZ_CP040543
• Organism: Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: FFT48_RS29870
• Protein ID: WP_071177730.1
• Coordinates: 7810..8124 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: FFT48_RS29875
• Protein ID: WP_000093040.1
• Coordinates: 8112..8390 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFT48_RS29845	3754..5718	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
FFT48_RS29850	5718..6449	+	732	WP_071177728.1	replication-relaxation family protein	-
FFT48_RS29855	6456..6986	+	531	WP_071177729.1	hypothetical protein	-
FFT48_RS29860	7013..7192	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
FFT48_RS29865	7218..7646	-	429	WP_001140599.1	hypothetical protein	-
FFT48_RS29870	7810..8124	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FFT48_RS29875	8112..8390	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
FFT48_RS30630	8724..8930	-	207	Protein_10	TonB family protein	-
FFT48_RS29885	8927..9298	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
FFT48_RS29890	9572..9817	-	246	WP_032440458.1	hypothetical protein	-
FFT48_RS29900	10462..11949	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T125978 WP_071177730.1 NZ_CP040543:c8124-7810 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T125978 NZ_CP040543:c8124-7810 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT125978 WP_000093040.1 NZ_CP040543:c8390-8112 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT125978 NZ_CP040543:c8390-8112 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1