125967

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 102486..103156 Replicon plasmid pCR-HvKP4-VIR
Accession NZ_CP040540
Organism Klebsiella pneumoniae strain CR-HvKP4

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag FFT48_RS27365 Protein ID WP_004213072.1
Coordinates 102486..102929 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag FFT48_RS27370 Protein ID WP_004213073.1
Coordinates 102926..103156 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFT48_RS27315 97894..98169 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
FFT48_RS27320 98232..98723 + 492 WP_011251327.1 DM13 domain-containing protein -
FFT48_RS27325 98772..99692 + 921 WP_004213934.1 DUF1471 domain-containing protein -
FFT48_RS27335 99783..100189 + 407 Protein_105 GAF domain-containing protein -
FFT48_RS30545 100703..101341 - 639 WP_032488580.1 RmpA2 -
FFT48_RS27355 101759..102052 + 294 Protein_107 transposase -
FFT48_RS27360 102056..102472 + 417 Protein_108 restriction endonuclease -
FFT48_RS27365 102486..102929 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
FFT48_RS27370 102926..103156 - 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FFT48_RS27375 103764..104897 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
FFT48_RS27380 104913..105206 + 294 WP_004213076.1 hypothetical protein -
FFT48_RS27385 105196..105402 - 207 WP_004213077.1 hypothetical protein -
FFT48_RS27390 105754..106044 + 291 WP_004213078.1 hypothetical protein -
FFT48_RS27395 106034..106933 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iucA / iucB / iucC / iucD / iutA / rmpA 1..178154 178154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T125967 WP_004213072.1 NZ_CP040540:c102929-102486 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T125967 NZ_CP040540:c102929-102486 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT125967 WP_004213073.1 NZ_CP040540:c103156-102926 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT125967 NZ_CP040540:c103156-102926 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q6U619


Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References