Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5312136..5312761 Replicon chromosome
Accession NZ_CP040533
Organism Klebsiella pneumoniae strain CR-HvKP1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FFT47_RS26345 Protein ID WP_002882817.1
Coordinates 5312136..5312519 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FFT47_RS26350 Protein ID WP_004150355.1
Coordinates 5312519..5312761 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFT47_RS26330 5309502..5310404 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FFT47_RS26335 5310401..5311036 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FFT47_RS26340 5311033..5311962 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FFT47_RS26345 5312136..5312519 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FFT47_RS26350 5312519..5312761 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FFT47_RS26355 5312966..5313883 + 918 WP_002882812.1 alpha/beta hydrolase -
FFT47_RS26360 5313897..5314838 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FFT47_RS26365 5314883..5315320 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FFT47_RS26370 5315317..5316177 - 861 WP_002882807.1 virulence factor BrkB family protein -
FFT47_RS26375 5316171..5316770 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T125932 WP_002882817.1 NZ_CP040533:c5312519-5312136 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T125932 NZ_CP040533:c5312519-5312136 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT125932 WP_004150355.1 NZ_CP040533:c5312761-5312519 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT125932 NZ_CP040533:c5312761-5312519 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References