Detailed information of TA system TA125885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2793523..2794159
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7J980
• Locus tag: FE661_RS14325
• Protein ID: WP_012537455.1
• Coordinates: 2793523..2793939 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J981
• Locus tag: FE661_RS14330
• Protein ID: WP_009560865.1
• Coordinates: 2793926..2794159 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS14290	2788703..2788855	-	153	WP_009566804.1	integrase core domain-containing protein	-
FE661_RS14295	2788862..2789149	-	288	WP_012537449.1	DDE-type integrase/transposase/recombinase	-
FE661_RS14300	2789512..2790438	-	927	WP_012537450.1	uracil-DNA glycosylase	-
FE661_RS14305	2790605..2791675	+	1071	WP_012537451.1	linear amide C-N hydrolase	-
FE661_RS14310	2791888..2792277	-	390	WP_111122584.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FE661_RS14315	2792268..2792519	-	252	WP_012537453.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FE661_RS14320	2792839..2793420	+	582	WP_146235847.1	hypothetical protein	-
FE661_RS14325	2793523..2793939	-	417	WP_012537455.1	PIN domain-containing protein	Toxin
FE661_RS14330	2793926..2794159	-	234	WP_009560865.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FE661_RS14335	2794158..2794418	+	261	WP_012607558.1	hypothetical protein	-
FE661_RS14340	2794515..2794865	+	351	WP_012537457.1	DUF4870 domain-containing protein	-
FE661_RS14345	2794962..2796824	+	1863	WP_012537458.1	ATP-binding cassette domain-containing protein	-
FE661_RS14350	2796821..2797144	-	324	WP_012537459.1	hypothetical protein	-
FE661_RS14355	2797137..2797448	-	312	WP_009566855.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15358.75 Da
• Isoelectric Point: 5.1352

>T125885 WP_012537455.1 NZ_CP040511:c2793939-2793523 [Acidithiobacillus ferrooxidans]
MASGKAFFDTNVLLYLLSADTAKADVAEGIMVNGGVISAQVLNEFASVAQRKMGMSFLEIREVLTPIHRICDVAHLTLDT
HIHGLRIAERYKYNVWDAMIVASALLADCAVLYTEDMHHGQILEDRLQLINPFLRADE

Nucleotide

• Length: 417 bp

>T125885 NZ_CP040511:c2793939-2793523 [Acidithiobacillus ferrooxidans]
ATGGCGAGCGGTAAGGCGTTCTTCGACACGAACGTGCTGCTTTATCTGCTTTCGGCGGACACGGCCAAGGCAGATGTTGC
AGAAGGCATCATGGTGAATGGTGGTGTGATCAGTGCCCAGGTGTTGAACGAATTTGCTTCCGTTGCCCAGCGCAAGATGG
GCATGTCTTTTCTGGAAATCAGAGAGGTGCTGACCCCCATTCATCGAATTTGCGATGTCGCGCATCTTACCCTGGATACG
CACATTCATGGCCTCAGGATTGCGGAGCGATACAAATACAATGTTTGGGATGCAATGATTGTCGCATCGGCCCTGTTGGC
TGATTGCGCCGTTCTTTACACGGAGGATATGCACCACGGCCAGATCCTCGAAGACCGCTTGCAACTGATCAATCCATTTT
TGCGGGCCGACGAGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8919.15 Da
• Isoelectric Point: 8.7371

>AT125885 WP_009560865.1 NZ_CP040511:c2794159-2793926 [Acidithiobacillus ferrooxidans]
MQVSKWGNSLAVRIPTSVVEALGIKEGDDIEVEVVGERRFQIQRTPQAEELLARLRRYRGRLPADFRFDRLEAHGER

Nucleotide

• Length: 234 bp

>AT125885 NZ_CP040511:c2794159-2793926 [Acidithiobacillus ferrooxidans]
ATGCAGGTATCCAAGTGGGGCAACAGTCTGGCCGTCCGTATACCAACCTCCGTGGTAGAGGCGCTGGGTATCAAAGAGGG
TGACGACATTGAGGTTGAGGTCGTGGGCGAACGCCGTTTTCAGATTCAACGAACGCCTCAAGCGGAAGAGTTGTTGGCCA
GGTTAAGGCGTTATCGAGGGAGGCTCCCTGCCGATTTCAGGTTCGACCGGTTGGAAGCGCATGGCGAGCGGTAA

Structures

Toxin

• AlphaFold DB: B7J980

Antitoxin

• AlphaFold DB: B7J981