Detailed information of TA system TA125884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 2293211..2293779
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5J6VSB9
• Locus tag: FE661_RS11670
• Protein ID: WP_012536619.1
• Coordinates: 2293405..2293779 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2Z6IL32
• Locus tag: FE661_RS11665
• Protein ID: WP_012536620.1
• Coordinates: 2293211..2293408 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS11620	2288510..2289406	+	897	WP_012536626.1	hypothetical protein	-
FE661_RS11625	2289431..2289700	+	270	WP_012536625.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FE661_RS11630	2289687..2289821	+	135	Protein_2284	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
FE661_RS11635	2290424..2290645	-	222	WP_009560828.1	HU family DNA-binding protein	-
FE661_RS11640	2290654..2291025	-	372	WP_180830528.1	hypothetical protein	-
FE661_RS11645	2291018..2291293	-	276	WP_012536624.1	HU family DNA-binding protein	-
FE661_RS11650	2291360..2291683	-	324	WP_180830446.1	integration host factor subunit alpha	-
FE661_RS11655	2291680..2292354	-	675	WP_012536622.1	hypothetical protein	-
FE661_RS11660	2292704..2292865	+	162	WP_012536621.1	hypothetical protein	-
FE661_RS11665	2293211..2293408	+	198	WP_012536620.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FE661_RS11670	2293405..2293779	+	375	WP_012536619.1	type II toxin-antitoxin system VapC family toxin	Toxin
FE661_RS11675	2293920..2294063	+	144	Protein_2293	SAM-dependent DNA methyltransferase	-
FE661_RS11680	2294181..2296979	+	2799	WP_012536617.1	class I SAM-dependent DNA methyltransferase	-
FE661_RS11685	2296976..2297673	+	698	Protein_2295	putative DNA binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-109)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13683.71 Da
• Isoelectric Point: 6.3157

>T125884 WP_012536619.1 NZ_CP040511:2293405-2293779 [Acidithiobacillus ferrooxidans]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSGLPRCTATSPEETLFFIGRHSLMGR
GIGYIDASILASAMLVQANLWSRDRRLMALATELGCAYSPTSSE

Nucleotide

• Length: 375 bp

>T125884 NZ_CP040511:2293405-2293779 [Acidithiobacillus ferrooxidans]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAGCAGACCTTATATCTGC
TCTCAGGCTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTTATTGGGCGTCATAGCCTGATGGGACGC
GGTATAGGCTATATTGACGCGAGCATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAGACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7266.37 Da
• Isoelectric Point: 9.4896

>AT125884 WP_012536620.1 NZ_CP040511:2293211-2293408 [Acidithiobacillus ferrooxidans]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA

Nucleotide

• Length: 198 bp

>AT125884 NZ_CP040511:2293211-2293408 [Acidithiobacillus ferrooxidans]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGCGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAAT
TACAGAACATTCCTCGCAGACGAGAGCCTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A5J6VSB9

Antitoxin

• AlphaFold DB: A0A2Z6IL32