Detailed information of TA system TA125883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1872963..1873600
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FE661_RS09590
• Protein ID: WP_012536995.1
• Coordinates: 1872963..1873373 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J4Y7
• Locus tag: FE661_RS09595
• Protein ID: WP_009567455.1
• Coordinates: 1873370..1873600 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS09570	1869543..1870586	-	1044	WP_012607324.1	phytoene/squalene synthase family protein	-
FE661_RS09575	1870745..1871779	-	1035	WP_012536992.1	NAD(P)-dependent glycerol-3-phosphate dehydrogenase	-
FE661_RS09580	1871783..1872232	-	450	WP_012536993.1	protein-export chaperone SecB	-
FE661_RS09590	1872963..1873373	-	411	WP_012536995.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FE661_RS09595	1873370..1873600	-	231	WP_009567455.1	antitoxin	Antitoxin
FE661_RS09600	1873903..1875423	-	1521	WP_012536996.1	aldehyde dehydrogenase	-
FE661_RS09605	1875726..1877438	+	1713	WP_009566247.1	proline--tRNA ligase	-
FE661_RS09610	1877431..1877907	+	477	WP_012536997.1	universal stress protein	-
FE661_RS09615	1877920..1878585	-	666	WP_012536998.1	membrane integrity-associated transporter subunit PqiC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15375.79 Da
• Isoelectric Point: 8.0756

>T125883 WP_012536995.1 NZ_CP040511:c1873373-1872963 [Acidithiobacillus ferrooxidans]
VIKYLLDTNICIFTIKNKPPEIREQFRQHHGQMAISTVTLMELIYGAEKSQVPERNLAEVEGFAARLEVLEFGSEAAKHT
GQLRAELAKIGKPIGPYDQMIAGHARSLGLFVVTNNLREFERVPGLRVMDWQSSRL

Nucleotide

• Length: 411 bp

>T125883 NZ_CP040511:c1873373-1872963 [Acidithiobacillus ferrooxidans]
GTGATCAAATACCTGCTGGATACCAATATCTGCATCTTTACTATCAAGAACAAGCCACCGGAGATCCGCGAACAGTTCCG
TCAGCACCATGGGCAAATGGCTATCAGCACCGTCACCTTGATGGAGTTGATCTATGGCGCGGAGAAATCACAGGTACCGG
AACGCAACCTGGCCGAAGTTGAAGGGTTCGCCGCCAGGCTGGAAGTGCTCGAATTCGGGAGCGAAGCCGCCAAGCATACT
GGGCAGCTCCGCGCAGAACTGGCCAAGATCGGCAAACCCATCGGGCCTTATGACCAGATGATCGCAGGCCACGCCAGAAG
CCTGGGATTGTTTGTCGTGACCAACAACTTGCGGGAATTCGAGCGCGTTCCGGGATTGCGGGTCATGGATTGGCAGAGCT
CACGCCTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8573.77 Da
• Isoelectric Point: 5.0508

>AT125883 WP_009567455.1 NZ_CP040511:c1873600-1873370 [Acidithiobacillus ferrooxidans]
MEHATVFQINRSQAIRLPKSIAFPADVKRVEVVAIGRMRILVPAGESWESWFDGEGVSADFMESREQPAGQFREPL

Nucleotide

• Length: 231 bp

>AT125883 NZ_CP040511:c1873600-1873370 [Acidithiobacillus ferrooxidans]
ATGGAGCATGCCACTGTTTTTCAGATCAACCGTAGCCAGGCGATACGTCTGCCCAAGTCGATAGCCTTTCCGGCAGATGT
GAAGCGCGTAGAGGTGGTCGCCATAGGCCGCATGCGCATTCTGGTTCCTGCCGGAGAGTCCTGGGAGAGCTGGTTCGATG
GAGAAGGGGTCAGCGCCGACTTCATGGAAAGCCGTGAGCAACCCGCCGGCCAGTTCCGGGAACCGTTGTGA

Structures

Antitoxin

• AlphaFold DB: B7J4Y7