Detailed information of TA system TA125882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1522964..1523638
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7JBN6
• Locus tag: FE661_RS07905
• Protein ID: WP_009564531.1
• Coordinates: 1523219..1523638 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7JBN5
• Locus tag: FE661_RS07900
• Protein ID: WP_012536752.1
• Coordinates: 1522964..1523218 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS07885	1518639..1519643	-	1005	WP_012536749.1	glycosyltransferase family 2 protein	-
FE661_RS07890	1519782..1521242	+	1461	WP_012536750.1	IMP dehydrogenase	-
FE661_RS07895	1521246..1522790	+	1545	WP_012536751.1	glutamine-hydrolyzing GMP synthase	-
FE661_RS07900	1522964..1523218	+	255	WP_012536752.1	plasmid stabilization protein	Antitoxin
FE661_RS07905	1523219..1523638	+	420	WP_009564531.1	type II toxin-antitoxin system VapC family toxin	Toxin
FE661_RS07910	1523656..1523868	+	213	WP_009564530.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FE661_RS07915	1523868..1523990	+	123	Protein_1556	VapC toxin family PIN domain ribonuclease	-
FE661_RS07920	1524703..1526322	+	1620	WP_012536754.1	APC family permease	-
FE661_RS07925	1526413..1527612	+	1200	WP_012536755.1	glutamate 5-kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14968.11 Da
• Isoelectric Point: 4.7051

>T125882 WP_009564531.1 NZ_CP040511:1523219-1523638 [Acidithiobacillus ferrooxidans]
MIVLDTNVVSEAMKPEPDAAVRAWLNEQAAETLYLSSVTLAELLFGIRALPAGKCKNMLDRALTELLELFKDRVLPFDTD
AARHYADLAVTAKNGGRGFPTPDGYIAAVAASRGFIVASRDTSPYEAAGVTVINPWKDA

Nucleotide

• Length: 420 bp

>T125882 NZ_CP040511:1523219-1523638 [Acidithiobacillus ferrooxidans]
ATGATCGTCCTGGATACCAACGTCGTTTCCGAGGCGATGAAGCCGGAGCCAGACGCGGCCGTGAGGGCTTGGCTGAACGA
ACAGGCGGCGGAAACCTTGTATCTGTCGAGCGTCACGCTGGCCGAGTTGCTGTTCGGCATCCGGGCGCTGCCGGCGGGCA
AGTGCAAGAACATGCTCGACCGCGCCCTGACCGAACTGCTGGAGCTGTTCAAGGATCGGGTGCTGCCCTTCGATACGGAT
GCAGCGCGCCACTATGCCGACCTGGCCGTGACGGCCAAGAACGGCGGGCGCGGCTTCCCGACGCCAGACGGCTACATCGC
GGCTGTTGCCGCATCGCGGGGCTTCATCGTGGCATCACGCGACACGTCGCCCTATGAAGCGGCCGGCGTGACCGTCATCA
ATCCCTGGAAGGATGCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9225.67 Da
• Isoelectric Point: 10.7239

>AT125882 WP_012536752.1 NZ_CP040511:1522964-1523218 [Acidithiobacillus ferrooxidans]
MAILTVRNVPDDVHRALRVRAARHGRSTEAEVREILAAAVKPSSRVCMGEALAAIGRKIGLTNEDFAVFESVRDKTPAKP
LRFD

Nucleotide

• Length: 255 bp

>AT125882 NZ_CP040511:1522964-1523218 [Acidithiobacillus ferrooxidans]
ATGGCAATCCTGACTGTAAGAAATGTGCCGGATGACGTGCATCGCGCTTTGCGCGTGCGGGCGGCCCGGCACGGTCGCAG
CACGGAAGCGGAGGTGCGCGAGATTCTGGCGGCTGCGGTCAAACCTTCGAGCCGCGTGTGCATGGGGGAAGCCCTGGCCG
CCATCGGCCGCAAGATCGGTCTGACCAATGAAGATTTCGCCGTTTTCGAGAGTGTGCGCGACAAGACGCCGGCCAAACCG
TTGCGCTTCGACTGA

Structures

Toxin

• AlphaFold DB: B7JBN6

Antitoxin

• AlphaFold DB: B7JBN5