Detailed information of TA system TA125881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1379498..1380261
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B7J9L5
• Locus tag: FE661_RS07150
• Protein ID: WP_009565062.1
• Coordinates: 1379498..1379992 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B7J9L6
• Locus tag: FE661_RS07155
• Protein ID: WP_012536505.1
• Coordinates: 1379989..1380261 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS07115	1375275..1376045	+	771	WP_012536502.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FE661_RS07120	1376038..1376880	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
FE661_RS07130	1377598..1378104	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	-
FE661_RS07135	1378101..1378430	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	-
FE661_RS07140	1378717..1378860	+	144	WP_009569579.1	hypothetical protein	-
FE661_RS07145	1378887..1379153	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
FE661_RS07150	1379498..1379992	-	495	WP_009565062.1	GNAT family N-acetyltransferase	Toxin
FE661_RS07155	1379989..1380261	-	273	WP_012536505.1	DUF1778 domain-containing protein	Antitoxin
FE661_RS07160	1380342..1380554	-	213	WP_009562915.1	hypothetical protein	-
FE661_RS07165	1380722..1380938	-	217	Protein_1407	hypothetical protein	-
FE661_RS07170	1381338..1382159	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
FE661_RS07175	1382400..1382645	+	246	WP_009568482.1	DUF2934 domain-containing protein	-
FE661_RS07180	1382840..1383976	+	1137	WP_012536507.1	glycosyltransferase family 1 protein	-
FE661_RS07185	1384010..1385224	+	1215	WP_012536508.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18013.83 Da
• Isoelectric Point: 10.4415

>T125881 WP_009565062.1 NZ_CP040511:c1379992-1379498 [Acidithiobacillus ferrooxidans]
MSLKLSAPQPLAAVHLLGDFESGEPALDEWLKRRAMNNQLTGASRTFVVVDEENRVRGFYAMAAGAVAHQVATSSVRRNM
PDPVPVMVLGRLAVDSRAQGIKLGAAMLQDAVKRAITVSQNTGVRALLVHALHERAKQFYEHYGFQESPQHPMTLMLRLN
SIKA

Nucleotide

• Length: 495 bp

>T125881 NZ_CP040511:c1379992-1379498 [Acidithiobacillus ferrooxidans]
ATGAGTTTGAAGCTATCCGCGCCGCAGCCACTTGCGGCCGTGCACCTGTTGGGCGACTTCGAGAGCGGTGAGCCTGCACT
GGATGAGTGGTTAAAACGCCGGGCGATGAACAACCAGCTCACCGGGGCCAGTCGAACCTTTGTGGTCGTTGATGAAGAAA
ATCGGGTGCGCGGCTTCTACGCCATGGCGGCTGGCGCAGTCGCACATCAAGTGGCGACTAGCTCGGTTCGTCGCAATATG
CCCGATCCGGTTCCCGTCATGGTGCTCGGTAGGTTGGCGGTGGATAGTCGAGCGCAAGGCATCAAGCTGGGGGCGGCCAT
GCTGCAGGACGCGGTGAAACGTGCCATCACCGTATCGCAGAACACGGGGGTTCGTGCACTGCTGGTTCATGCGCTCCACG
AACGCGCCAAACAGTTCTATGAGCACTACGGCTTTCAGGAGTCACCACAACATCCGATGACGCTGATGTTGCGCCTGAAT
AGCATCAAGGCTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10071.52 Da
• Isoelectric Point: 5.0914

>AT125881 WP_012536505.1 NZ_CP040511:c1380261-1379989 [Acidithiobacillus ferrooxidans]
MRAAAINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVLLDQVFFGLDAEKFRQFTAMLDAPPRGNEGLERLMA
VKAPWESNQA

Nucleotide

• Length: 273 bp

>AT125881 NZ_CP040511:c1380261-1379989 [Acidithiobacillus ferrooxidans]
ATGCGTGCCGCTGCCATCAATTTACGAGCCTTGCCGGAGCAGCGTGATCTCATTGATCACGCCGCCAACCTGTTAGGCAA
AAACCGTTCGGACTTCATGCTGGAAGCCGCCTGCGAGCGTGCGCAATCGGTACTGCTCGATCAGGTGTTTTTTGGGCTGG
ATGCGGAAAAGTTCCGGCAGTTCACCGCCATGCTCGACGCACCGCCTCGTGGCAACGAAGGTCTTGAGCGTCTGATGGCA
GTCAAGGCGCCATGGGAAAGCAATCAAGCATGA

Structures

Toxin

• AlphaFold DB: B7J9L5

Antitoxin

• AlphaFold DB: B7J9L6