Detailed information of TA system TA125879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 1353626..1354245
• Replicon: chromosome
• Accession: NZ_CP040511
• Organism: Acidithiobacillus ferrooxidans strain YNTRS-40

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FE661_RS06995
• Protein ID: WP_054608979.1
• Coordinates: 1353626..1353979 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2W1K4M0
• Locus tag: FE661_RS07000
• Protein ID: WP_054608978.1
• Coordinates: 1353976..1354245 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE661_RS06970	1350163..1350570	-	408	WP_054608983.1	hypothetical protein	-
FE661_RS06975	1350567..1351043	-	477	WP_055448961.1	hypothetical protein	-
FE661_RS06980	1351037..1352446	-	1410	WP_055448960.1	hypothetical protein	-
FE661_RS06985	1352716..1353123	-	408	WP_054608981.1	transcriptional regulator	-
FE661_RS06990	1353123..1353413	-	291	WP_054608980.1	type II toxin-antitoxin system HigB family toxin	-
FE661_RS06995	1353626..1353979	-	354	WP_054608979.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FE661_RS07000	1353976..1354245	-	270	WP_054608978.1	hypothetical protein	Antitoxin
FE661_RS07005	1354394..1354645	+	252	WP_054608977.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
FE661_RS07015	1355103..1356239	+	1137	WP_012536490.1	FIST C-terminal domain-containing protein	-
FE661_RS07020	1356342..1358075	+	1734	WP_012607053.1	thiol reductant ABC exporter subunit CydD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12842.63 Da
• Isoelectric Point: 7.8845

>T125879 WP_054608979.1 NZ_CP040511:c1353979-1353626 [Acidithiobacillus ferrooxidans]
MIGRKIPSPGDVYWINPNPTAGNEIRDRHRFVVITPTEINKLGLVTCVPITSGGNYARQNGLTVVIAGHDTTGVAVCNQM
RTFDLHARAGDVKYVETLDQQTTREIINRVTSIIDPA

Nucleotide

• Length: 354 bp

>T125879 NZ_CP040511:c1353979-1353626 [Acidithiobacillus ferrooxidans]
TTGATCGGACGGAAGATTCCATCGCCCGGCGACGTGTACTGGATCAATCCCAACCCAACAGCAGGAAACGAAATCCGGGA
CCGGCACAGGTTCGTGGTCATCACACCCACCGAGATCAACAAGCTGGGCCTGGTAACGTGTGTACCCATCACCAGCGGCG
GCAATTACGCCAGGCAGAACGGATTGACCGTGGTCATCGCGGGGCACGACACGACGGGAGTGGCCGTCTGCAATCAAATG
CGTACCTTCGACCTGCACGCCAGAGCGGGGGACGTGAAGTACGTGGAGACGTTGGACCAGCAAACCACGCGCGAAATCAT
CAACCGGGTAACCAGCATCATCGATCCAGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9708.11 Da
• Isoelectric Point: 5.1053

>AT125879 WP_054608978.1 NZ_CP040511:c1354245-1353976 [Acidithiobacillus ferrooxidans]
MLVTRIRKQGGAAVVTLPAEVLRQLDAGIGEDLSIEVSQHTVIMRPARGERRRYNLEELLAGVTGQDMQILAGDTAWARE
GDPAGRELI

Nucleotide

• Length: 270 bp

>AT125879 NZ_CP040511:c1354245-1353976 [Acidithiobacillus ferrooxidans]
ATGTTGGTCACAAGGATTCGCAAGCAAGGCGGTGCGGCTGTGGTTACGCTCCCCGCGGAAGTGTTGAGGCAGTTGGATGC
CGGCATCGGTGAGGATTTGAGCATCGAGGTCTCCCAGCACACGGTTATCATGCGTCCAGCACGCGGTGAGCGACGGCGCT
ACAACCTGGAAGAATTGCTCGCTGGCGTCACCGGGCAGGACATGCAGATCCTCGCCGGTGATACCGCTTGGGCCAGAGAA
GGCGATCCCGCCGGGAGAGAGTTGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W1K4M0