Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-doc
Location 28766..29351 Replicon plasmid pM190262
Accession NZ_CP040462
Organism Enterococcus sp. M190262

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag FE005_RS15855 Protein ID WP_138372245.1
Coordinates 28986..29351 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag FE005_RS15850 Protein ID WP_138372244.1
Coordinates 28766..28996 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE005_RS15820 23877..24188 - 312 WP_138372240.1 PadR family transcriptional regulator -
FE005_RS15825 24627..25265 - 639 WP_138372241.1 hypothetical protein -
FE005_RS15830 26212..26583 + 372 WP_138372242.1 hypothetical protein -
FE005_RS15835 26600..27094 + 495 Protein_25 hypothetical protein -
FE005_RS15840 27111..27551 + 441 WP_138372243.1 hypothetical protein -
FE005_RS16900 28092..28658 + 567 WP_175413580.1 hypothetical protein -
FE005_RS15850 28766..28996 + 231 WP_138372244.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FE005_RS15855 28986..29351 + 366 WP_138372245.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FE005_RS15860 29887..30579 + 693 WP_138372246.1 N-6 DNA methylase -
FE005_RS15865 30640..31086 + 447 WP_175413581.1 hypothetical protein -
FE005_RS15870 31498..31965 - 468 WP_138372247.1 single-stranded DNA-binding protein -
FE005_RS15875 31993..34131 - 2139 WP_138372248.1 DUF3991 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..211104 211104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 14060.10 Da        Isoelectric Point: 9.6564

>T125869 WP_138372245.1 NZ_CP040462:28986-29351 [Enterococcus sp. M190262]
MNYKPRQRDIVILDFEPSKGYEVRKRRPALVLSKDTYNSATNLIIVCPITTSDKERPFLVPIHSDKLKSNDSKPSKVNTL
QVFSLDYTQAANRNVKYVDTLDEEVFFDIAQRFLKNFSFPI

Download         Length: 366 bp

>T125869 NZ_CP040462:28986-29351 [Enterococcus sp. M190262]
ATGAACTATAAACCACGACAGCGAGACATTGTCATTCTTGATTTTGAGCCCTCAAAAGGCTATGAAGTTAGGAAACGACG
TCCTGCCTTAGTTTTGAGTAAAGATACCTATAATTCTGCAACGAATCTAATCATTGTTTGCCCAATCACTACAAGTGACA
AAGAACGTCCATTTCTTGTCCCTATTCACAGTGACAAGCTCAAATCAAACGATTCGAAGCCTAGCAAGGTAAACACACTT
CAAGTATTCTCACTTGATTACACCCAGGCAGCCAACCGAAACGTCAAGTATGTGGATACCCTTGACGAAGAAGTATTTTT
TGACATTGCACAGAGATTTCTAAAAAACTTTTCCTTTCCTATTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8309.52 Da        Isoelectric Point: 4.4436

>AT125869 WP_138372244.1 NZ_CP040462:28766-28996 [Enterococcus sp. M190262]
MASTKTRKQGNSLVITIPAKLGLKEGEEFLIVKKENGTVALIPKVEDFFESAKEGEFSLPELAIDYTPQGGELNEL

Download         Length: 231 bp

>AT125869 NZ_CP040462:28766-28996 [Enterococcus sp. M190262]
ATGGCTTCTACAAAAACCAGAAAACAAGGAAATTCCCTTGTCATCACGATTCCTGCTAAGCTAGGACTCAAAGAAGGCGA
AGAATTTCTAATCGTCAAAAAGGAAAATGGAACGGTCGCTCTCATTCCCAAAGTTGAAGATTTTTTTGAATCTGCCAAAG
AAGGTGAGTTTTCTCTTCCTGAATTAGCAATCGATTATACGCCCCAGGGAGGCGAACTGAATGAACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References