Detailed information of TA system TA125853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2582747..2584335
• Replicon: chromosome
• Accession: NZ_CP040456
• Organism: Escherichia coli strain UPEC132

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: FE252_RS14045
• Protein ID: WP_001125475.1
• Coordinates: 2583013..2584335 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FE252_RS14040
• Protein ID: WP_001332145.1
• Coordinates: 2582747..2583013 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE252_RS14025	2579115..2580566	+	1452	WP_000854636.1	tagaturonate reductase	-
FE252_RS14030	2580763..2581677	+	915	WP_001306069.1	bestrophin family protein	-
FE252_RS14035	2581681..2582439	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
FE252_RS14040	2582747..2583013	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FE252_RS14045	2583013..2584335	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FE252_RS14055	2584594..2585255	-	662	Protein_2403	alpha/beta hydrolase	-
FE252_RS14060	2585244..2585723	+	480	WP_015912499.1	cupin domain-containing protein	-
FE252_RS14065	2585701..2586462	+	762	WP_001066622.1	carboxymuconolactone decarboxylase family protein	-
FE252_RS14070	2586620..2587684	+	1065	WP_000859696.1	alpha/beta fold hydrolase	-
FE252_RS14080	2587933..2588244	-	312	WP_001468159.1	transcriptional regulator	-
FE252_RS14090	2588542..2589324	+	783	WP_000699097.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T125853 WP_001125475.1 NZ_CP040456:2583013-2584335 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T125853 NZ_CP040456:2583013-2584335 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT125853 WP_001332145.1 NZ_CP040456:2582747-2583013 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT125853 NZ_CP040456:2582747-2583013 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8