125836

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 105529..106064 Replicon plasmid unnamed1
Accession NZ_CP040454
Organism Escherichia coli strain UPEC132

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag FE252_RS00745 Protein ID WP_001270417.1
Coordinates 105777..106064 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag FE252_RS00740 Protein ID WP_001132895.1
Coordinates 105529..105780 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE252_RS00695 100615..100857 - 243 WP_113365707.1 hypothetical protein -
FE252_RS00705 100921..101280 - 360 WP_119159666.1 DUF1281 domain-containing protein -
FE252_RS00710 101287..101430 - 144 WP_000270807.1 DUF1281 domain-containing protein -
FE252_RS00720 101597..102373 - 777 Protein_117 hypothetical protein -
FE252_RS00725 102607..103569 - 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
FE252_RS00730 103566..104771 - 1206 WP_000817635.1 AAA family ATPase -
FE252_RS00740 105529..105780 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FE252_RS00745 105777..106064 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FE252_RS00750 106063..106311 + 249 Protein_122 integrase core domain-containing protein -
FE252_RS00765 106990..107967 + 978 WP_021515438.1 RepB family plasmid replication initiator protein -
FE252_RS00770 108246..108986 - 741 WP_139510873.1 tyrosine-type recombinase/integrase -
FE252_RS00775 109107..109295 - 189 WP_032144810.1 hypothetical protein -
FE252_RS00780 109667..110836 + 1170 WP_001072359.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..117139 117139


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T125836 WP_001270417.1 NZ_CP040454:105777-106064 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T125836 NZ_CP040454:105777-106064 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT125836 WP_001132895.1 NZ_CP040454:105529-105780 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT125836 NZ_CP040454:105529-105780 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References