Detailed information of TA system TA125829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 35370..35929
• Replicon: plasmid unnamed1
• Accession: NZ_CP040454
• Organism: Escherichia coli strain UPEC132

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A376ZSB3
• Locus tag: FE252_RS00170
• Protein ID: WP_021515398.1
• Coordinates: 35370..35645 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FE252_RS00175
• Protein ID: WP_024187480.1
• Coordinates: 35645..35929 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE252_RS00155	32674..33450	-	777	WP_021515395.1	energy transducer TonB	-
FE252_RS00160	33458..34333	-	876	WP_021515396.1	ChaN family lipoprotein	-
FE252_RS00165	34610..35104	+	495	Protein_28	IS91 family transposase	-
FE252_RS00170	35370..35645	-	276	WP_021515398.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE252_RS00175	35645..35929	-	285	WP_024187480.1	ribbon-helix-helix domain-containing protein	Antitoxin
FE252_RS00185	36477..36905	-	429	WP_021515400.1	Exc2 family lipoprotein	-
FE252_RS00195	37357..38004	+	648	WP_021515401.1	hypothetical protein	-
FE252_RS00200	38229..38528	-	300	WP_021515402.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FE252_RS00205	38518..38781	-	264	WP_001089474.1	hypothetical protein	-
FE252_RS00210	38888..39148	-	261	WP_024187481.1	hypothetical protein	-
FE252_RS00225	39851..40708	-	858	WP_145747938.1	incFII family plasmid replication initiator RepA	-
FE252_RS00230	40701..40775	-	75	WP_001365705.1	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..117139	117139
	-	flank	IS/Tn	-	-	34610..35104	494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10778.55 Da
• Isoelectric Point: 10.2344

>T125829 WP_021515398.1 NZ_CP040454:c35645-35370 [Escherichia coli]
MELKWTSKALSNLARLYDFLVLASKPAAARMVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T125829 NZ_CP040454:c35645-35370 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTAATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAATGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10470.89 Da
• Isoelectric Point: 5.6558

>AT125829 WP_024187480.1 NZ_CP040454:c35929-35645 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT125829 NZ_CP040454:c35929-35645 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCACTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A376ZSB3