Detailed information of TA system TA125821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4658980..4660568
• Replicon: chromosome
• Accession: NZ_CP040443
• Organism: Escherichia sp. E4742

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FEM44_RS22650
• Protein ID: WP_138159200.1
• Coordinates: 4658980..4660302 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FEM44_RS22655
• Protein ID: WP_130219125.1
• Coordinates: 4660302..4660568 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM44_RS22630	4654233..4655783	-	1551	WP_135522419.1	site-specific integrase	-
FEM44_RS22635	4655767..4656642	-	876	WP_001059075.1	site-specific integrase	-
FEM44_RS22645	4657442..4658839	+	1398	WP_135522418.1	ISNCY family transposase	-
FEM44_RS22650	4658980..4660302	-	1323	WP_138159200.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FEM44_RS22655	4660302..4660568	-	267	WP_130219125.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FEM44_RS22660	4660942..4662534	-	1593	WP_135522417.1	autoinducer-2 kinase	-
FEM44_RS22665	4662613..4663566	-	954	WP_135522416.1	transcriptional regulator LsrR	-
FEM44_RS22670	4663814..4665349	+	1536	WP_135522415.1	autoinducer 2 ABC transporter ATP-binding protein LsrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4657442..4658839	1397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49262.79 Da
• Isoelectric Point: 7.8729

>T125821 WP_138159200.1 NZ_CP040443:c4660302-4658980 [Escherichia sp. E4742]
MPKLVTWMNNQRVGELTKLANGAHTFRYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPVVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPEDETVTHPIMAWEKLTDARLEEVLTAYKADIPLGMIREENDFGISVAGAQEKTAL
LRMGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTILLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGRKTTIDKIYPRHFLATAKALKFPEVQMHEILSDFASMIP
AALDNVKTSLPADFPENVVTAIETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T125821 NZ_CP040443:c4660302-4658980 [Escherichia sp. E4742]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTGGCCAATGGCGCGCACACTTTTAG
GTATGCGCCGGAGTGGTTAGCCAACCGTTATGCCAGGCCTTTGTCGCTATCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTGTTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGGTTGTACGCGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGCGCCGTGACATTATTACCAGAAGA
TGAAACCGTAACGCACCCGATAATGGCATGGGAAAAGCTTACTGACGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATTCCGCTAGGCATGATAAGAGAAGAAAATGACTTTGGCATCTCGGTTGCTGGCGCGCAGGAGAAGACCGCGCTG
CTCAGAATGGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCATATCATTAAATTACCGATTGGTGAAAT
CAGGCAGCCCAACGCGACGCTCGATCTCAGTCAAAGCGTGGATAATGAATATTACTGTCTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATAAAAGCGGGTAGAGTGCGCGCGTTAGCGGTCGAACGCTTTGACAGGCGCTGG
AATACTGAACGAACGATTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCTGACGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTAATGGGTTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTATTCCAGTGGTTGATTGGCGCGACGGACGGCCATGCGAAAAATTTTTCGGTTTTTATCCAG
GCCGGTGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTCCCGGTATTGGGTGGCGCGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAGAAAAACGACAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGCGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGCATGATTCCC
GCAGCACTGGATAACGTGAAGACTTCATTACCGGCAGATTTTCCGGAGAATGTGGTGACGGCAATTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGAATTAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9985.36 Da
• Isoelectric Point: 4.6081

>AT125821 WP_130219125.1 NZ_CP040443:c4660568-4660302 [Escherichia sp. E4742]
MISFPMIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNAAPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT125821 NZ_CP040443:c4660568-4660302 [Escherichia sp. E4742]
ATGATAAGCTTTCCGATGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
ACAGAGCGAGCTGGCGAAAAAAATCGGCATCAAGCAGGCGACGATTTCGAATTTCGAGAATAATCCCGATAACACCACGC
TGACGACATTTTTTAAGATATTACAGTCACTTGAACTCTCAATGACGCTATGCGACACGAAAAACGCTGCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA