Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3476800..3477418 Replicon chromosome
Accession NZ_CP040443
Organism Escherichia sp. E4742

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag FEM44_RS16840 Protein ID WP_001280991.1
Coordinates 3476800..3477018 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FEM44_RS16845 Protein ID WP_000344800.1
Coordinates 3477044..3477418 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEM44_RS16805 3472095..3472667 + 573 WP_135523138.1 YbaY family lipoprotein -
FEM44_RS16810 3472698..3473009 - 312 WP_171022606.1 MGMT family protein -
FEM44_RS16820 3473389..3473742 + 354 WP_064529090.1 DUF1428 domain-containing protein -
FEM44_RS16825 3473780..3475330 - 1551 WP_130258550.1 EAL domain-containing protein -
FEM44_RS16830 3475493..3475963 - 471 WP_130206287.1 YlaC family protein -
FEM44_RS16835 3476077..3476628 - 552 WP_135523136.1 maltose O-acetyltransferase -
FEM44_RS16840 3476800..3477018 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
FEM44_RS16845 3477044..3477418 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FEM44_RS16850 3477976..3481125 - 3150 WP_130223199.1 efflux RND transporter permease AcrB -
FEM44_RS16855 3481148..3482341 - 1194 WP_130215483.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T125816 WP_001280991.1 NZ_CP040443:c3477018-3476800 [Escherichia sp. E4742]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T125816 NZ_CP040443:c3477018-3476800 [Escherichia sp. E4742]
ATGTCCGATAAACCTTTAACGAAAACCGATTATTTGATGCGTCTGCGTCGTTGCCAGACAATTGACACGCTTGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCAGAAT
TGACCATGAATAAACTGTACGACAAGATCCCCTCCTCTGTATGGAAATTTATTCGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT125816 WP_000344800.1 NZ_CP040443:c3477418-3477044 [Escherichia sp. E4742]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT125816 NZ_CP040443:c3477418-3477044 [Escherichia sp. E4742]
ATGGATGAATACTCACCAAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCTTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGTAACCATGGTTGGGTTAACGACCCTACCTCGGCCATCAATCTGCAGCTTAATGAACTCATTGAGC
ATATAGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATAGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTCCAGAAATGGCGGAAGTCAGGTAACCGACT
GTTCCGTTGTTTTGTGAATGCCACTAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References