Detailed information of TA system TA125812

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2569770..2570266
• Replicon: chromosome
• Accession: NZ_CP040443
• Organism: Escherichia sp. E4742

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: A0A5F1G8P7
• Locus tag: FEM44_RS12470
• Protein ID: WP_064525730.1
• Coordinates: 2570093..2570266 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: FEM44_RS12465
• Protein ID: WP_130205929.1
• Coordinates: 2569770..2570066 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM44_RS12445	2566511..2567230	-	720	WP_073511157.1	two-component system response regulator DcuR	-
FEM44_RS12450	2567227..2568858	-	1632	WP_135523565.1	sensor histidine kinase	-
FEM44_RS12455	2569038..2569268	+	231	WP_135523566.1	(4Fe-4S)-binding protein	-
FEM44_RS12460	2569280..2569552	+	273	WP_000405647.1	N-acetyltransferase	-
FEM44_RS12465	2569770..2570066	+	297	WP_130205929.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
FEM44_RS12470	2570093..2570266	+	174	WP_064525730.1	type V toxin-antitoxin system toxin GhoT	Toxin
FEM44_RS12475	2570385..2571902	-	1518	WP_135523567.1	lysine--tRNA ligase	-
FEM44_RS12480	2572139..2573596	-	1458	WP_135523568.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2569770..2579116	9346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (7-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6585.09 Da
• Isoelectric Point: 10.2288

>T125812 WP_064525730.1 NZ_CP040443:2570093-2570266 [Escherichia sp. E4742]
MTLFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T125812 NZ_CP040443:2570093-2570266 [Escherichia sp. E4742]
ATGACACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTGGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11468.06 Da
• Isoelectric Point: 4.4287

>AT125812 WP_130205929.1 NZ_CP040443:2569770-2570066 [Escherichia sp. E4742]
MEGKKKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRIKCLDLPDI
DFDLNIMIVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT125812 NZ_CP040443:2569770-2570066 [Escherichia sp. E4742]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTCGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTTTTCTTAAGGTT
AAGATCATTGATGCATGATATGAACTTCTCCTCTATAGTGGCTGATGAATATGGAATACCACGACAATTGAATGAGAATT
CTTTCGCAATAACGACATCATTAGCCGCAAGTGAAATTGAAGATTTAATCAGGATCAAATGCTTAGACTTGCCAGATATT
GATTTTGACCTCAACATTATGATAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Structures

Toxin

• AlphaFold DB: A0A5F1G8P7