Detailed information of TA system TA125811

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2129524..2130359
• Replicon: chromosome
• Accession: NZ_CP040443
• Organism: Escherichia sp. E4742

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: L4IWC0
• Locus tag: FEM44_RS10380
• Protein ID: WP_001604178.1
• Coordinates: 2129524..2129901 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FEM44_RS10385
• Protein ID: WP_135523322.1
• Coordinates: 2129991..2130359 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM44_RS10355	2125159..2126061	-	903	WP_135523326.1	carboxylate/amino acid/amine transporter	-
FEM44_RS10360	2126266..2127483	+	1218	WP_135523325.1	flagellin lysine-N-methylase	-
FEM44_RS10365	2127892..2128737	-	846	WP_135523372.1	DUF4942 domain-containing protein	-
FEM44_RS10370	2128822..2129019	-	198	WP_135523324.1	DUF957 domain-containing protein	-
FEM44_RS10375	2129039..2129527	-	489	WP_135523323.1	hypothetical protein	-
FEM44_RS10380	2129524..2129901	-	378	WP_001604178.1	TA system toxin CbtA family protein	Toxin
FEM44_RS10385	2129991..2130359	-	369	WP_135523322.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FEM44_RS10390	2130439..2130660	-	222	WP_135523321.1	DUF987 domain-containing protein	-
FEM44_RS10395	2130729..2131205	-	477	WP_001384029.1	RadC family protein	-
FEM44_RS10400	2131221..2131700	-	480	WP_135524061.1	antirestriction protein	-
FEM44_RS10410	2132019..2132837	-	819	WP_135524062.1	DUF945 domain-containing protein	-
FEM44_RS10420	2132927..2133160	-	234	WP_024193317.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14325.35 Da
• Isoelectric Point: 7.8046

>T125811 WP_001604178.1 NZ_CP040443:c2129901-2129524 [Escherichia sp. E4742]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
STCTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHTEVKR

Nucleotide

• Length: 378 bp

>T125811 NZ_CP040443:c2129901-2129524 [Escherichia sp. E4742]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGAATTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATACTGAGGTGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13574.44 Da
• Isoelectric Point: 6.4757

>AT125811 WP_135523322.1 NZ_CP040443:c2130359-2129991 [Escherichia sp. E4742]
VSDILSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPHHQHTVTLYARGLTCAADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT125811 NZ_CP040443:c2130359-2129991 [Escherichia sp. E4742]
GTGTCAGACATACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGTCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCGGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCACCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGCAGCCGATACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: L4IWC0