Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 918029..918683 Replicon chromosome
Accession NZ_CP040443
Organism Escherichia sp. E4742

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag FEM44_RS04350 Protein ID WP_135487323.1
Coordinates 918029..918436 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FEM44_RS04355 Protein ID WP_000354046.1
Coordinates 918417..918683 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEM44_RS04330 913986..915719 - 1734 WP_135519883.1 single-stranded-DNA-specific exonuclease RecJ -
FEM44_RS04335 915725..916435 - 711 WP_130207826.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FEM44_RS04340 916460..917356 - 897 WP_064529792.1 site-specific tyrosine recombinase XerD -
FEM44_RS04345 917468..917989 + 522 WP_130207828.1 flavodoxin FldB -
FEM44_RS04350 918029..918436 - 408 WP_135487323.1 protein YgfX Toxin
FEM44_RS04355 918417..918683 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FEM44_RS04360 918935..919915 + 981 WP_135521122.1 tRNA-modifying protein YgfZ -
FEM44_RS04365 920101..920760 - 660 WP_135521120.1 hemolysin III family protein -
FEM44_RS04370 920924..921235 - 312 WP_135521118.1 N(4)-acetylcytidine aminohydrolase -
FEM44_RS04375 921280..922713 + 1434 WP_138158872.1 6-phospho-beta-glucosidase BglA -
FEM44_RS04380 922775..923518 - 744 WP_135521114.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16076.01 Da        Isoelectric Point: 11.5202

>T125808 WP_135487323.1 NZ_CP040443:c918436-918029 [Escherichia sp. E4742]
VVLWQSDLRVSWRAQWLSLLIHGLVAAIILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T125808 NZ_CP040443:c918436-918029 [Escherichia sp. E4742]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGTTGGTTGCCGC
TATTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTGCTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAGATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGAATTTTGTTGCAACAAGAGACGC
AAAGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT125808 WP_000354046.1 NZ_CP040443:c918683-918417 [Escherichia sp. E4742]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT125808 NZ_CP040443:c918683-918417 [Escherichia sp. E4742]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGCGACGACCCGGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References