Detailed information of TA system TA125780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2674957..2675485
• Replicon: chromosome
• Accession: NZ_CP040428
• Organism: Jejubacter calystegiae strain KSNA2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: FEM41_RS12240
• Protein ID: WP_138096241.1
• Coordinates: 2675177..2675485 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: FEM41_RS12235
• Protein ID: WP_138096240.1
• Coordinates: 2674957..2675175 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM41_RS12215	2670109..2670666	+	558	WP_138096236.1	HTH-type transcriptional regulator PuuR	-
FEM41_RS12220	2670802..2672292	+	1491	WP_138096237.1	aldehyde dehydrogenase PuuC	-
FEM41_RS12225	2672295..2673575	+	1281	WP_138096238.1	FAD-binding oxidoreductase	-
FEM41_RS12230	2673750..2674835	+	1086	WP_138096239.1	malate/lactate/ureidoglycolate dehydrogenase	-
FEM41_RS12235	2674957..2675175	+	219	WP_138096240.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
FEM41_RS12240	2675177..2675485	+	309	WP_138096241.1	type II toxin-antitoxin system toxin CcdB	Toxin
FEM41_RS12245	2675527..2676870	-	1344	WP_138096242.1	NCS2 family permease	-
FEM41_RS12250	2676851..2678683	-	1833	WP_138096243.1	adenine deaminase	-
FEM41_RS12255	2678811..2679722	+	912	WP_138096244.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11856.63 Da
• Isoelectric Point: 7.0088

>T125780 WP_138096241.1 NZ_CP040428:2675177-2675485 [Jejubacter calystegiae]
MQYRVYAYKRKESRYRLFVDVQSDIVDTPGRRMVIPLVSASLLSERVSRELYPVVRFDGDDYRLMTTDMASVTIAVTGRE
LADLSHYENAIKDAINLMFWGI

Nucleotide

• Length: 309 bp

>T125780 NZ_CP040428:2675177-2675485 [Jejubacter calystegiae]
ATGCAGTACCGTGTCTATGCCTATAAGCGGAAGGAGAGCCGCTACCGCCTGTTCGTCGACGTCCAGAGCGATATAGTGGA
TACCCCAGGCCGAAGAATGGTGATCCCATTGGTTAGCGCCAGCCTGCTGTCAGAGCGCGTTTCCCGTGAACTGTATCCGG
TGGTTCGATTCGACGGTGATGACTACCGACTAATGACCACTGATATGGCCAGCGTGACCATCGCCGTGACTGGCCGGGAA
CTGGCCGATCTCAGCCATTATGAGAATGCGATTAAGGATGCGATAAATCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8310.22 Da
• Isoelectric Point: 4.7954

>AT125780 WP_138096240.1 NZ_CP040428:2674957-2675175 [Jejubacter calystegiae]
MKQRITVTVDSDHYQQLKEAGVNISGLVSAAMEQEARRLRAERWRAENLEGMAEVARFIEQNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT125780 NZ_CP040428:2674957-2675175 [Jejubacter calystegiae]
ATGAAACAGCGGATCACCGTGACCGTCGACAGCGATCACTACCAGCAGCTTAAGGAGGCCGGGGTCAATATCTCAGGACT
GGTCAGCGCCGCCATGGAACAGGAAGCCCGGCGGTTGCGCGCCGAACGCTGGCGGGCCGAAAACCTTGAAGGGATGGCCG
AGGTCGCCCGCTTTATCGAACAGAATGGCTCGTTTGCCGATGAAAACAGGGACTGGTGA