Detailed information of TA system TA125776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 326381..327024
• Replicon: chromosome
• Accession: NZ_CP040428
• Organism: Jejubacter calystegiae strain KSNA2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FEM41_RS01320
• Protein ID: WP_138093677.1
• Coordinates: 326608..327024 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: FEM41_RS01315
• Protein ID: WP_138093674.1
• Coordinates: 326381..326611 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM41_RS01300	322416..324479	-	2064	WP_138093668.1	dimethylglycine demethylation protein DgcA	-
FEM41_RS01305	324537..325067	-	531	WP_138093671.1	4-vinyl reductase	-
FEM41_RS01310	325089..326066	-	978	WP_138099078.1	dipeptidase	-
FEM41_RS01315	326381..326611	+	231	WP_138093674.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FEM41_RS01320	326608..327024	+	417	WP_138093677.1	type II toxin-antitoxin system VapC family toxin	Toxin
FEM41_RS01325	327069..328223	-	1155	WP_138093680.1	GlxA family transcriptional regulator	-
FEM41_RS01330	328510..329886	-	1377	WP_138093683.1	L-serine ammonia-lyase	-
FEM41_RS01335	330174..331829	+	1656	WP_138093686.1	BCCT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15173.59 Da
• Isoelectric Point: 6.7051

>T125776 WP_138093677.1 NZ_CP040428:326608-327024 [Jejubacter calystegiae]
MTKTYMLDTCICSYIMREQPEAVIRRLEQAVMQRHRIVVSAITYAEMRFGTLGKKASPRHAQLVEAFCIRLDAVMPWDRA
AVDETTELKATLTAAGTPIGPNDTAIAGHAIAAGAILVTNNVKEFERVPGLTLEDWAA

Nucleotide

• Length: 417 bp

>T125776 NZ_CP040428:326608-327024 [Jejubacter calystegiae]
ATGACCAAAACCTATATGCTGGACACCTGTATCTGCTCTTATATCATGCGTGAGCAGCCGGAAGCCGTGATTCGGCGTCT
GGAGCAGGCGGTCATGCAGCGTCATCGCATTGTGGTGTCGGCTATTACCTATGCCGAAATGCGCTTCGGTACCCTCGGTA
AAAAAGCGTCGCCGCGCCATGCGCAGCTGGTAGAGGCCTTCTGTATCCGTCTTGACGCTGTTATGCCGTGGGACCGCGCC
GCAGTGGATGAAACCACTGAGCTGAAAGCAACGCTTACCGCCGCCGGTACGCCCATCGGGCCCAACGATACGGCCATTGC
CGGGCATGCCATCGCCGCTGGCGCCATCCTTGTCACCAATAATGTGAAGGAATTTGAGCGGGTGCCGGGATTAACCCTGG
AAGACTGGGCCGCATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8791.96 Da
• Isoelectric Point: 4.5230

>AT125776 WP_138093674.1 NZ_CP040428:326381-326611 [Jejubacter calystegiae]
MRTVSVFKNGNNRAIRLPRDLDFEGVNELEITREGDTILLRPIKPSWGSFLLEEKADADFMTEREEIVNDEGRVKL

Nucleotide

• Length: 231 bp

>AT125776 NZ_CP040428:326381-326611 [Jejubacter calystegiae]
ATGAGAACGGTATCTGTGTTTAAAAACGGCAATAACCGCGCGATCCGCTTACCCAGGGATCTGGATTTTGAGGGCGTTAA
CGAACTGGAGATTACCCGTGAAGGCGACACTATCCTTCTGCGGCCGATCAAACCGAGCTGGGGATCGTTCCTGCTGGAAG
AGAAAGCCGATGCGGACTTCATGACGGAACGTGAAGAGATTGTGAATGACGAGGGGCGCGTTAAGCTATGA