Detailed information of TA system TA125770

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii(toxin)
• Location: 1171248..1172680
• Replicon: chromosome
• Accession: NZ_CP040424
• Organism: Dolosigranulum pigrum strain KPL3033

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: -
• Locus tag: FE321_RS05395
• Protein ID: WP_208954842.1
• Coordinates: 1171835..1172680 (+)
• Length: 282 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: -
• Locus tag: FE321_RS05390
• Protein ID: WP_208955560.1
• Coordinates: 1171248..1171838 (+)
• Length: 197 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE321_RS05370 (FE321_05345)	1167373..1169667	-	2295	WP_208954840.1	hypothetical protein	-
FE321_RS05375 (FE321_05350)	1169751..1170089	-	339	WP_208954505.1	TrbC/VirB2 family protein	-
FE321_RS05380 (FE321_05355)	1170172..1170471	-	300	WP_208954504.1	hypothetical protein	-
FE321_RS05385 (FE321_05360)	1170598..1170813	-	216	WP_208954841.1	hypothetical protein	-
FE321_RS05390 (FE321_05365)	1171248..1171838	+	591	WP_208955560.1	abortive phage infection protein	Antitoxin
FE321_RS05395 (FE321_05370)	1171835..1172680	+	846	WP_208954842.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
FE321_RS05400 (FE321_05375)	1172873..1173484	-	612	WP_244359434.1	Clp protease ClpP	-
FE321_RS05405 (FE321_05380)	1173474..1174682	-	1209	Protein_1072	phage portal protein	-
FE321_RS05410 (FE321_05385)	1174796..1175524	-	729	WP_111974701.1	DUF4065 domain-containing protein	-
FE321_RS05415 (FE321_05390)	1175535..1176119	-	585	WP_111974700.1	hypothetical protein	-
FE321_RS05420 (FE321_05395)	1176293..1176745	+	453	WP_111974699.1	transposase	-
FE321_RS05425 (FE321_05400)	1176929..1177678	-	750	WP_208954843.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1170598..1199716	29118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-266)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 282 a.a.
• Molecular weight: 32513.13 Da
• Isoelectric Point: 6.8373

>T125770 WP_208954842.1 NZ_CP040424:1171835-1172680 [Dolosigranulum pigrum]
MNKAKLTALCHKISKQTGLTFNSVMTYYFLEVILKKLSHSPYSKHYIFKGGFLLSNVIGVNARSTVDIDFLFHRITLSEE
TIRQQLKDILTDSNDEILFVIKSIATIKENDEYSGYRVSILCQLENIKQTIHLDIATGDIVTPHPITYDYKAIFDDENFP
ITAYTIETILAEKLQTIYSRSFLNSRSKDFYDIYILVKLKKDAIGFSQFQTACTKTFSARGTELNYNKIIELLNKFKTDA
IQNQQWQNYSKKYSYTKDIEFSDVLDEIINLLYAINSSDDH

Nucleotide

• Length: 846 bp

>T125770 NZ_CP040424:1171835-1172680 [Dolosigranulum pigrum]
ATGAACAAAGCAAAACTAACAGCGCTCTGCCACAAAATCTCAAAACAAACAGGCTTAACTTTCAACTCTGTTATGACTTA
TTATTTTCTTGAGGTTATTTTAAAAAAATTAAGTCATAGCCCTTATTCAAAACATTACATTTTCAAAGGCGGATTTTTAC
TCTCCAATGTTATTGGTGTCAATGCACGGAGTACCGTTGATATTGATTTTCTCTTTCATCGAATAACACTCTCAGAAGAA
ACTATTCGGCAACAACTAAAAGACATCTTAACTGATTCAAACGATGAAATCTTATTTGTGATTAAGTCCATTGCTACCAT
CAAAGAAAATGATGAATATAGTGGCTATCGAGTATCTATTCTCTGTCAGCTTGAAAATATAAAACAAACCATACACCTAG
ACATTGCAACTGGTGATATTGTGACTCCTCATCCGATCACTTATGATTACAAAGCTATTTTTGATGATGAAAATTTCCCA
ATCACTGCTTATACGATCGAAACTATTCTTGCTGAAAAACTACAAACTATTTATTCTAGAAGTTTTTTGAATAGTCGCAG
TAAAGATTTTTATGATATTTATATTCTTGTAAAATTAAAGAAAGATGCAATTGGTTTTTCTCAATTCCAGACAGCTTGCA
CAAAAACATTTTCTGCTCGTGGCACTGAACTGAATTACAATAAAATCATCGAGTTACTAAATAAATTTAAGACTGACGCC
ATTCAAAATCAACAATGGCAAAATTATTCTAAAAAATATAGCTATACAAAAGATATCGAATTCTCTGATGTTCTTGACGA
AATCATTAACTTATTATACGCCATCAATTCTTCAGATGATCATTAA

Antitoxin

Protein

• Length: 197 a.a.
• Molecular weight: 23124.30 Da
• Isoelectric Point: 5.1086

>AT125770 WP_208955560.1 NZ_CP040424:1171248-1171838 [Dolosigranulum pigrum]
ITKKQKLLHYLEENNGIITHQDCQKLDIPTVYLTRLEKNGTIFRVEQGIYLTPSGDYDEYYFFQYRFSKAIFSYISALYL
QGLTDEIPQYFEVTVARGYRFNNPPSNLNIHFVSKEYSSLGITSVETPMENTVQVYDIERTLCDFIINREKIDSELFVKT
LQLYADYPKKNTAKLYEYATEMHIIEETKRTLEVLI

Nucleotide

• Length: 591 bp

>AT125770 NZ_CP040424:1171248-1171838 [Dolosigranulum pigrum]
ATAACTAAAAAACAAAAGCTACTCCATTATCTAGAAGAAAATAATGGGATCATAACACACCAAGATTGTCAGAAACTCGA
TATCCCGACAGTATACCTGACAAGACTAGAGAAGAATGGGACAATTTTCCGCGTTGAACAAGGCATTTACCTGACCCCTA
GTGGTGATTACGATGAATATTATTTCTTTCAATATCGCTTTTCCAAGGCTATCTTTTCCTACATTTCTGCTCTTTATCTT
CAAGGCCTTACTGATGAAATTCCGCAATATTTTGAAGTCACTGTCGCAAGAGGCTACCGATTTAATAATCCACCATCAAA
TCTAAACATTCATTTTGTGTCTAAAGAATATAGTTCTCTCGGAATAACAAGCGTAGAAACTCCTATGGAAAATACTGTAC
AAGTTTACGACATTGAACGTACTCTGTGTGATTTTATCATCAATCGAGAAAAAATCGACTCTGAACTTTTTGTCAAAACA
CTTCAACTTTATGCAGATTATCCTAAGAAGAACACGGCAAAGCTCTATGAATACGCTACAGAAATGCATATTATAGAAGA
GACTAAACGAACACTGGAGGTACTCATATGA