Detailed information of TA system TA125769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 705963..706453
• Replicon: chromosome
• Accession: NZ_CP040424
• Organism: Dolosigranulum pigrum strain KPL3033

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H3NFC0
• Locus tag: FE321_RS03390
• Protein ID: WP_004636454.1
• Coordinates: 706187..706453 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FE321_RS03385
• Protein ID: WP_208955434.1
• Coordinates: 705963..706190 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE321_RS03375 (FE321_03350)	701147..703000	+	1854	WP_208955433.1	ABC transporter substrate-binding protein	-
FE321_RS03380 (FE321_03355)	703077..705629	-	2553	WP_004636456.1	transglycosylase domain-containing protein	-
FE321_RS03385 (FE321_03360)	705963..706190	+	228	WP_208955434.1	DUF6290 family protein	Antitoxin
FE321_RS03390 (FE321_03365)	706187..706453	+	267	WP_004636454.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE321_RS03395 (FE321_03370)	706564..707529	-	966	WP_111950672.1	rhodanese-related sulfurtransferase	-
FE321_RS03400 (FE321_03375)	707665..709470	-	1806	WP_208955435.1	M3 family oligoendopeptidase	-
FE321_RS03405 (FE321_03380)	709625..710092	-	468	WP_208955436.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10367.24 Da
• Isoelectric Point: 10.5009

>T125769 WP_004636454.1 NZ_CP040424:706187-706453 [Dolosigranulum pigrum]
MSYQLMLSNKARKQLKKLDPPVAEMIIRWLKANVDGVDNPRKHGKALTGEYKDLWRYRVGNYRIICDVRDRELIILALAV
GHRKDIYK

Nucleotide

• Length: 267 bp

>T125769 NZ_CP040424:706187-706453 [Dolosigranulum pigrum]
ATGAGCTACCAGTTAATGTTAAGCAACAAAGCTAGAAAGCAACTTAAAAAGTTAGACCCTCCCGTAGCTGAGATGATTAT
ACGGTGGTTGAAAGCTAATGTGGATGGTGTAGATAATCCTAGAAAGCATGGTAAGGCACTGACAGGGGAATATAAAGACT
TATGGCGTTATCGTGTAGGGAATTATCGTATTATTTGTGATGTGAGAGATAGAGAACTAATTATATTAGCATTAGCAGTT
GGACATCGAAAAGATATTTACAAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8491.58 Da
• Isoelectric Point: 3.9947

>AT125769 WP_208955434.1 NZ_CP040424:705963-706190 [Dolosigranulum pigrum]
MTIVSVRLNKEEEQVINGYALLTGQPISQLFKQALIREIEDQLDYKGGIKALTEHEDDPQTFTLEDMADELGINL

Nucleotide

• Length: 228 bp

>AT125769 NZ_CP040424:705963-706190 [Dolosigranulum pigrum]
ATGACAATTGTATCTGTACGACTGAATAAAGAAGAAGAGCAAGTAATTAATGGATATGCCTTATTGACAGGTCAACCGAT
ATCACAATTATTTAAGCAAGCGTTGATTAGAGAGATTGAAGATCAACTAGACTACAAGGGGGGGATTAAAGCGTTAACTG
AGCACGAGGATGACCCTCAGACATTTACCTTAGAGGATATGGCAGATGAGTTAGGAATTAATTTATGA

Structures

Toxin

• AlphaFold DB: A0A1S8KMK3