Detailed information of TA system TA125757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 723663..724153
• Replicon: chromosome
• Accession: NZ_CP040409
• Organism: Dolosigranulum pigrum strain KPL3274

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H3NFC0
• Locus tag: FE336_RS03395
• Protein ID: WP_004636454.1
• Coordinates: 723887..724153 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FE336_RS03390
• Protein ID: WP_208956276.1
• Coordinates: 723663..723890 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE336_RS03380 (FE336_03365)	718847..720700	+	1854	WP_208956274.1	oligopeptide ABC transporter substrate-binding protein	-
FE336_RS03385 (FE336_03370)	720777..723329	-	2553	WP_208956275.1	transglycosylase domain-containing protein	-
FE336_RS03390 (FE336_03375)	723663..723890	+	228	WP_208956276.1	DUF6290 family protein	Antitoxin
FE336_RS03395 (FE336_03380)	723887..724153	+	267	WP_004636454.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE336_RS03400 (FE336_03385)	724264..725229	-	966	WP_111950672.1	rhodanese-related sulfurtransferase	-
FE336_RS03405 (FE336_03390)	725365..727170	-	1806	WP_208956277.1	M3 family oligoendopeptidase	-
FE336_RS03410 (FE336_03395)	727325..727792	-	468	WP_208956278.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10367.24 Da
• Isoelectric Point: 10.5009

>T125757 WP_004636454.1 NZ_CP040409:723887-724153 [Dolosigranulum pigrum]
MSYQLMLSNKARKQLKKLDPPVAEMIIRWLKANVDGVDNPRKHGKALTGEYKDLWRYRVGNYRIICDVRDRELIILALAV
GHRKDIYK

Nucleotide

• Length: 267 bp

>T125757 NZ_CP040409:723887-724153 [Dolosigranulum pigrum]
ATGAGCTACCAGTTAATGTTAAGCAACAAAGCTAGAAAGCAACTTAAAAAGTTAGACCCTCCCGTAGCTGAGATGATTAT
ACGGTGGTTGAAAGCTAATGTGGATGGTGTAGATAATCCTAGAAAGCATGGTAAGGCACTGACAGGGGAATATAAAGATT
TATGGCGTTATCGTGTAGGGAATTATCGTATTATTTGTGATGTGAGAGATAGAGAACTAATTATATTAGCATTAGCAGTT
GGACATCGAAAAGATATTTACAAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8503.64 Da
• Isoelectric Point: 3.9947

>AT125757 WP_208956276.1 NZ_CP040409:723663-723890 [Dolosigranulum pigrum]
MTIVSVRLNKEEEQVINGYALLTGQPISQLFKQALIREIEDQLDYKVGIKALAEHEDDPQTFTLEDMADELGINL

Nucleotide

• Length: 228 bp

>AT125757 NZ_CP040409:723663-723890 [Dolosigranulum pigrum]
ATGACAATTGTATCTGTACGACTGAATAAAGAAGAAGAGCAAGTAATTAATGGATATGCCTTATTGACAGGTCAACCGAT
ATCACAATTATTTAAGCAAGCGTTGATTAGAGAGATTGAAGATCAACTAGACTACAAGGTGGGGATTAAAGCGTTAGCTG
AGCACGAGGATGACCCTCAGACATTTACCTTAGAGGATATGGCAGATGAGTTAGGAATTAATTTATGA

Structures

Toxin

• AlphaFold DB: A0A1S8KMK3