Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 55702..56303 Replicon plasmid pCTX-M-15_22372
Accession NZ_CP040398
Organism Escherichia coli strain BA22372

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag FEV15_RS23580 Protein ID WP_001216034.1
Coordinates 55702..56082 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag FEV15_RS23585 Protein ID WP_001190712.1
Coordinates 56082..56303 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEV15_RS23535 51044..51351 + 308 Protein_61 type II toxin-antitoxin system ParD family antitoxin -
FEV15_RS23545 51694..51876 + 183 WP_042065278.1 hypothetical protein -
FEV15_RS23550 51997..52737 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -
FEV15_RS23555 53022..53998 - 977 Protein_64 RepB family plasmid replication initiator protein -
FEV15_RS23565 54794..55207 - 414 Protein_65 DDE-type integrase/transposase/recombinase -
FEV15_RS23570 55131..55490 - 360 WP_001513660.1 hypothetical protein -
FEV15_RS23575 55518..55697 - 180 WP_001513661.1 hypothetical protein -
FEV15_RS23580 55702..56082 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FEV15_RS23585 56082..56303 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FEV15_RS23590 56486..58042 + 1557 WP_001553856.1 type I restriction-modification system subunit M -
FEV15_RS23595 58039..59322 + 1284 WP_001617890.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId / aadA5 / qacE / sul1 / tet(B) - 1..98470 98470
- flank IS/Tn - - 54794..55168 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T125749 WP_001216034.1 NZ_CP040398:c56082-55702 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T125749 NZ_CP040398:c56082-55702 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT125749 WP_001190712.1 NZ_CP040398:c56303-56082 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT125749 NZ_CP040398:c56303-56082 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References