Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4491705..4492359 Replicon chromosome
Accession NZ_CP040397
Organism Escherichia coli strain BA22372

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FEV15_RS21875 Protein ID WP_000244777.1
Coordinates 4491705..4492112 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FEV15_RS21880 Protein ID WP_000354046.1
Coordinates 4492093..4492359 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEV15_RS21855 4487662..4489395 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
FEV15_RS21860 4489401..4490111 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FEV15_RS21865 4490136..4491032 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FEV15_RS21870 4491144..4491665 + 522 WP_001055874.1 flavodoxin FldB -
FEV15_RS21875 4491705..4492112 - 408 WP_000244777.1 toxin CptA Toxin
FEV15_RS21880 4492093..4492359 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FEV15_RS21885 4492602..4493582 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FEV15_RS21890 4493659..4494318 - 660 WP_000250274.1 hemolysin III family protein -
FEV15_RS21895 4494482..4494793 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
FEV15_RS21900 4494838..4496271 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FEV15_RS21905 4496328..4497071 - 744 Protein_4218 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T125746 WP_000244777.1 NZ_CP040397:c4492112-4491705 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T125746 NZ_CP040397:c4492112-4491705 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT125746 WP_000354046.1 NZ_CP040397:c4492359-4492093 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT125746 NZ_CP040397:c4492359-4492093 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References