Detailed information of TA system TA125743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4050779..4051404
• Replicon: chromosome
• Accession: NZ_CP040397
• Organism: Escherichia coli strain BA22372

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FEV15_RS19750
• Protein ID: WP_000911330.1
• Coordinates: 4050779..4051177 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FEV15_RS19755
• Protein ID: WP_000450524.1
• Coordinates: 4051177..4051404 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEV15_RS19730	4046497..4047242	+	746	Protein_3802	IS21-like element ISEc12 family helper ATPase IstB	-
FEV15_RS19735	4047324..4047665	-	342	Protein_3803	esterase	-
FEV15_RS19740	4047739..4049754	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FEV15_RS19745	4049769..4050632	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
FEV15_RS19750	4050779..4051177	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FEV15_RS19755	4051177..4051404	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FEV15_RS19760	4051558..4052271	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FEV15_RS19765	4052484..4053518	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FEV15_RS19770	4053535..4054413	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FEV15_RS19775	4054559..4055131	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FEV15_RS19780	4055131..4055601	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T125743 WP_000911330.1 NZ_CP040397:c4051177-4050779 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T125743 NZ_CP040397:c4051177-4050779 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT125743 WP_000450524.1 NZ_CP040397:c4051404-4051177 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT125743 NZ_CP040397:c4051404-4051177 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33