Detailed information of TA system TA125728

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 584827..585628
• Replicon: chromosome
• Accession: NZ_CP040397
• Organism: Escherichia coli strain BA22372

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: FEV15_RS02890
• Protein ID: WP_001094437.1
• Coordinates: 585251..585628 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: FEV15_RS02885
• Protein ID: WP_001390338.1
• Coordinates: 584827..585204 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEV15_RS02840	579902..580471	-	570	WP_000148641.1	inovirus Gp2 family protein	-
FEV15_RS02855	581215..581426	-	212	Protein_550	hemolysin expression modulator Hha	-
FEV15_RS02860	581816..582580	+	765	WP_162869019.1	GTPase family protein	-
FEV15_RS02865	582952..583716	+	765	Protein_552	autotransporter adhesin family protein	-
FEV15_RS02870	583718..583888	+	171	Protein_553	antirestriction protein	-
FEV15_RS02875	583904..584380	+	477	WP_001186747.1	RadC family protein	-
FEV15_RS02880	584443..584664	+	222	WP_000692345.1	DUF987 domain-containing protein	-
FEV15_RS02885	584827..585204	+	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FEV15_RS02890	585251..585628	+	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
FEV15_RS02895	585625..586113	+	489	WP_000761716.1	hypothetical protein	-
FEV15_RS02900	586133..586330	+	198	WP_000772032.1	DUF957 domain-containing protein	-
FEV15_RS02905	586415..587260	+	846	WP_001274561.1	DUF4942 domain-containing protein	-
FEV15_RS02910	587495..587623	+	129	Protein_561	virulence RhuM family protein	-
FEV15_RS02915	588053..589237	+	1185	WP_001172877.1	sugar efflux transporter	-
FEV15_RS02920	589348..590271	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T125728 WP_001094437.1 NZ_CP040397:585251-585628 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T125728 NZ_CP040397:585251-585628 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT125728 WP_001390338.1 NZ_CP040397:584827-585204 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT125728 NZ_CP040397:584827-585204 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1