Detailed information of TA system TA125725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 26228..26750
• Replicon: plasmid pKrDSM107547
• Accession: NZ_CP040393
• Organism: Kosakonia radicincitans strain DSM 107547

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: FEI17_RS26985
• Protein ID: WP_004187019.1
• Coordinates: 26228..26512 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: FEI17_RS26990
• Protein ID: WP_004187025.1
• Coordinates: 26502..26750 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEI17_RS26965	21301..23307	+	2007	WP_149289414.1	ParB/RepB/Spo0J family partition protein	-
FEI17_RS26970	23351..23782	+	432	WP_149289415.1	conjugation system SOS inhibitor PsiB	-
FEI17_RS26975	23779..24513	+	735	WP_149289416.1	plasmid SOS inhibition protein A	-
FEI17_RS26980	24869..26212	+	1344	WP_149289417.1	ISNCY family transposase	-
FEI17_RS26985	26228..26512	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEI17_RS26990	26502..26750	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
FEI17_RS26995	27279..28169	-	891	Protein_29	transposase	-
FEI17_RS27000	28252..29274	+	1023	WP_149288265.1	IS21-like element ISSen3 family transposase	-
FEI17_RS27005	29271..30053	+	783	WP_149288264.1	IS21-like element ISSen3 family helper ATPase IstB	-
FEI17_RS27010	30135..30683	-	549	Protein_32	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118312	118312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T125725 WP_004187019.1 NZ_CP040393:c26512-26228 [Kosakonia radicincitans]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T125725 NZ_CP040393:c26512-26228 [Kosakonia radicincitans]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTGCAGGTGCAATTCAA
AAAGAAGCTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATACCGCCTTGTCTATCGTGTAGAGGACGACATTGTTACCGTCACTGTTATCGGTGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT125725 WP_004187025.1 NZ_CP040393:c26750-26502 [Kosakonia radicincitans]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT125725 NZ_CP040393:c26750-26502 [Kosakonia radicincitans]
ATGGCATTTCAGGTTCTGACCACTACGGCAGCCAGCATTACTGAGCTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTGAATCGTAATGAGCCTGCATTCTACTGCGTGCCACCGGCACTGTATGCCCAGTTGA
TGGAACTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4