Detailed information of TA system TA125723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4826703..4827157
• Replicon: chromosome
• Accession: NZ_CP040392
• Organism: Kosakonia radicincitans strain DSM 107547

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: FEI17_RS22920
• Protein ID: WP_007373008.1
• Coordinates: 4827023..4827157 (-)
• Length: 45 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: FEI17_RS22915
• Protein ID: WP_149289188.1
• Coordinates: 4826703..4827008 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEI17_RS22900	4823137..4825290	+	2154	WP_007373012.1	pyruvate/proton symporter BtsT	-
FEI17_RS22905	4825445..4825648	+	204	WP_035886582.1	YbdD/YjiX family protein	-
FEI17_RS22910	4825659..4826615	+	957	WP_149289187.1	GTPase	-
FEI17_RS22915	4826703..4827008	-	306	WP_149289188.1	BrnA antitoxin family protein	Antitoxin
FEI17_RS22920	4827023..4827157	-	135	WP_007373008.1	BrnT family toxin	Toxin
FEI17_RS22925	4827284..4828654	-	1371	WP_007373007.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
FEI17_RS22930	4828853..4829374	+	522	WP_149289189.1	VOC family protein	-
FEI17_RS22935	4829376..4830479	-	1104	WP_007373005.1	M20 peptidase aminoacylase family protein	-
FEI17_RS22940	4830504..4830971	-	468	WP_007373004.1	YjiG family protein	-
FEI17_RS22945	4830964..4831647	-	684	WP_007373003.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-41)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 5328.82 Da
• Isoelectric Point: 4.6615

>T125723 WP_007373008.1 NZ_CP040392:c4827157-4827023 [Kosakonia radicincitans]
MEFEWDVTKARSNQRKHGIRFEEAVSVFEDPYHLSIQDRFENGE

Nucleotide

• Length: 135 bp

>T125723 NZ_CP040392:c4827157-4827023 [Kosakonia radicincitans]
ATGGAGTTTGAATGGGACGTCACTAAAGCAAGAAGTAATCAACGCAAGCATGGCATTCGCTTTGAAGAAGCCGTATCGGT
TTTCGAGGATCCTTACCATTTGTCCATCCAGGACCGGTTCGAGAATGGCGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11505.28 Da
• Isoelectric Point: 10.3140

>AT125723 WP_149289188.1 NZ_CP040392:c4827008-4826703 [Kosakonia radicincitans]
MSMVKHKSGELPALNKQREAELKALANKSDGEIDYSDIPSTDDVLWAGAVRSKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMMRDLQNKK

Nucleotide

• Length: 306 bp

>AT125723 NZ_CP040392:c4827008-4826703 [Kosakonia radicincitans]
ATGAGCATGGTTAAACATAAATCGGGTGAGTTGCCAGCGCTCAACAAGCAGCGTGAAGCTGAATTAAAAGCGCTGGCAAA
TAAATCGGACGGAGAGATTGATTACAGCGATATTCCGTCCACAGATGACGTGTTATGGGCTGGCGCTGTGCGCAGTAAGT
TTTTTCGTCCGTTAAAAACGCAGGCATCAGTGCGTATTGATGCTGATGTGATGGAGTGGTTAAAACGACCGGGGAAAGGG
TATCAGACGCGGCTCAATGCCATTTTGCGTGAGGCAATGATGCGCGATTTGCAGAACAAGAAGTAA