Detailed information of TA system TA125720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3976866..3977660
• Replicon: chromosome
• Accession: NZ_CP040392
• Organism: Kosakonia radicincitans strain DSM 107547

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: FEI17_RS18860
• Protein ID: WP_149289012.1
• Coordinates: 3977139..3977660 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: FEI17_RS18855
• Protein ID: WP_007373817.1
• Coordinates: 3976866..3977135 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEI17_RS18830	3973027..3973641	+	615	WP_072438404.1	methylthioribulose 1-phosphate dehydratase	-
FEI17_RS18835	3973638..3974327	+	690	WP_072438402.1	acireductone synthase	-
FEI17_RS18840	3974324..3974866	+	543	WP_007373820.1	acireductone dioxygenase	-
FEI17_RS18845	3975001..3975828	-	828	WP_149289011.1	hypothetical protein	-
FEI17_RS27600	3975927..3976100	-	174	WP_167287342.1	hypothetical protein	-
FEI17_RS18850	3976343..3976660	+	318	WP_064567913.1	hypothetical protein	-
FEI17_RS18855	3976866..3977135	+	270	WP_007373817.1	DUF1778 domain-containing protein	Antitoxin
FEI17_RS18860	3977139..3977660	+	522	WP_149289012.1	GNAT family N-acetyltransferase	Toxin
FEI17_RS18865	3977694..3977921	-	228	WP_035886083.1	helix-turn-helix domain-containing protein	-
FEI17_RS18870	3978172..3978744	+	573	WP_149289013.1	DUF1349 domain-containing protein	-
FEI17_RS18875	3979175..3980713	+	1539	WP_007373813.1	MCP four helix bundle domain-containing protein	-
FEI17_RS18880	3980798..3981058	-	261	WP_035886084.1	MafI family immunity protein	-
FEI17_RS18885	3981058..3981387	-	330	WP_149289014.1	SdpI family protein	-
FEI17_RS18890	3981617..3982390	-	774	WP_007373810.1	hypothetical protein	-
FEI17_RS18895	3982393..3982590	-	198	WP_007373809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (93-157)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19515.40 Da
• Isoelectric Point: 8.4708

>T125720 WP_149289012.1 NZ_CP040392:3977139-3977660 [Kosakonia radicincitans]
VDNLTIEMFSGETEYETGGFDCGEVSLNAFLTNHLKRQHDGKILRAWLLVTKTPRPRILGYYTLSGGSFEKEQLPTKTQQ
KKVPYHNVPCVILGRLTIDKSLQGNGWGETLVAHAMRVVYNASLTIGIHGLFVEALTGNAKKFYEKLGFIPLADRNQHSL
FFPTKSIERLFEE

Nucleotide

• Length: 522 bp

>T125720 NZ_CP040392:3977139-3977660 [Kosakonia radicincitans]
GTGGACAACCTGACCATTGAGATGTTCTCAGGAGAGACAGAGTATGAAACAGGAGGTTTTGATTGTGGTGAGGTATCGCT
CAACGCTTTTTTAACCAACCATCTCAAACGCCAGCATGATGGCAAAATTTTACGTGCCTGGCTCCTTGTAACGAAAACGC
CCAGGCCGCGCATTTTAGGCTATTACACCTTGTCTGGCGGCAGTTTCGAGAAAGAACAACTGCCCACGAAAACACAGCAA
AAAAAGGTGCCTTATCACAATGTCCCCTGCGTCATATTAGGTCGGCTCACTATTGATAAGTCACTCCAGGGAAACGGCTG
GGGAGAAACCCTGGTGGCGCACGCCATGAGGGTGGTTTACAACGCTTCACTTACCATCGGCATACATGGGTTATTTGTCG
AAGCACTCACTGGCAACGCCAAAAAATTCTATGAAAAGCTGGGCTTCATTCCTTTAGCCGATCGCAATCAGCATTCGCTG
TTCTTTCCAACAAAGTCTATTGAACGCCTGTTCGAAGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10273.77 Da
• Isoelectric Point: 7.0179

>AT125720 WP_007373817.1 NZ_CP040392:3976866-3977135 [Kosakonia radicincitans]
MPALKKQRIDLRLTDEDKKMIEEAAAMTNQTVTQFLVNSASERAAEVIEQHRRLILSEESWNRVMDAIENPPAPNDKLKR
AAKRLQNME

Nucleotide

• Length: 270 bp

>AT125720 NZ_CP040392:3976866-3977135 [Kosakonia radicincitans]
ATGCCCGCACTGAAAAAACAGCGCATAGATCTGCGACTAACGGATGAGGATAAGAAAATGATCGAAGAAGCGGCGGCAAT
GACCAATCAGACGGTCACACAGTTTCTGGTTAACAGCGCTTCTGAACGCGCCGCGGAAGTGATTGAACAGCACCGTCGCC
TGATTCTCAGCGAGGAATCATGGAACAGGGTTATGGATGCTATCGAAAACCCACCAGCACCGAATGACAAACTTAAACGC
GCCGCAAAACGTCTGCAAAACATGGAGTGA