Detailed information of TA system TA125692

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 714270..715111
• Replicon: chromosome
• Accession: NZ_CP040391
• Organism: Klebsiella pneumoniae strain LSH-KPN25

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0WJM1
• Locus tag: FEF28_RS03565
• Protein ID: WP_000854822.1
• Coordinates: 714728..715111 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0CWL2
• Locus tag: FEF28_RS03560
• Protein ID: WP_053271974.1
• Coordinates: 714270..714638 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEF28_RS03540	712015..712833	+	819	WP_001234702.1	DUF945 domain-containing protein	-
FEF28_RS03545	712924..713409	+	486	WP_000214317.1	antirestriction protein	-
FEF28_RS03550	713424..713900	+	477	WP_001186747.1	RadC family protein	-
FEF28_RS03555	713969..714190	+	222	WP_000692312.1	DUF987 domain-containing protein	-
FEF28_RS03560	714270..714638	+	369	WP_053271974.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FEF28_RS03565	714728..715111	+	384	WP_000854822.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FEF28_RS03570	715102..715590	+	489	WP_001177592.1	hypothetical protein	-
FEF28_RS03575	715557..715799	+	243	WP_166635458.1	DUF957 domain-containing protein	-
FEF28_RS03580	715896..716741	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
FEF28_RS03590	717040..717546	+	507	WP_002917636.1	G/U mismatch-specific DNA glycosylase	-
FEF28_RS03595	717646..719487	-	1842	WP_004174339.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	floR / sul2 / aph(4)-Ia / aac(3)-IVa / dfrA12 / aadA2 / qacE / sul1	-	665077..716741	51664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14295.32 Da
• Isoelectric Point: 8.2830

>T125692 WP_000854822.1 NZ_CP040391:714728-715111 [Klebsiella pneumoniae]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHQESKRCN

Nucleotide

• Length: 384 bp

>T125692 NZ_CP040391:714728-715111 [Klebsiella pneumoniae]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13679.41 Da
• Isoelectric Point: 6.4758

>AT125692 WP_053271974.1 NZ_CP040391:714270-714638 [Klebsiella pneumoniae]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADANHLDQAFPLLMKQLELMLTSS
ELNPHRQNTVTLYVKGLTCHADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT125692 NZ_CP040391:714270-714638 [Klebsiella pneumoniae]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A1M0WJM1

Antitoxin

• AlphaFold DB: A0A1M0CWL2