Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3361839..3362457 Replicon chromosome
Accession NZ_CP040390
Organism Escherichia coli strain A1_136

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FE198_RS16635 Protein ID WP_001291435.1
Coordinates 3361839..3362057 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FE198_RS16640 Protein ID WP_000344800.1
Coordinates 3362083..3362457 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE198_RS16600 3357128..3357700 + 573 WP_000779831.1 YbaY family lipoprotein -
FE198_RS16605 3357731..3358042 - 312 WP_000409911.1 MGMT family protein -
FE198_RS16615 3358421..3358774 + 354 WP_001550741.1 DUF1428 family protein -
FE198_RS16620 3358816..3360366 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FE198_RS16625 3360530..3361000 - 471 WP_000136192.1 YlaC family protein -
FE198_RS16630 3361116..3361667 - 552 WP_060579167.1 maltose O-acetyltransferase -
FE198_RS16635 3361839..3362057 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FE198_RS16640 3362083..3362457 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FE198_RS16645 3363003..3366152 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
FE198_RS16650 3366175..3367368 - 1194 WP_138585050.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T125676 WP_001291435.1 NZ_CP040390:c3362057-3361839 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T125676 NZ_CP040390:c3362057-3361839 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT125676 WP_000344800.1 NZ_CP040390:c3362457-3362083 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT125676 NZ_CP040390:c3362457-3362083 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References