125673

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2729575..2730359 Replicon chromosome
Accession NZ_CP040390
Organism Escherichia coli strain A1_136

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag FE198_RS13535 Protein ID WP_000613626.1
Coordinates 2729575..2730069 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag FE198_RS13540 Protein ID WP_001110447.1
Coordinates 2730066..2730359 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE198_RS13515 2724768..2725865 + 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -
FE198_RS13520 2725865..2726806 + 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
FE198_RS13525 2726872..2728515 + 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
FE198_RS13530 2728527..2729480 + 954 WP_001212768.1 flagellar hook-associated protein FlgL -
FE198_RS13535 2729575..2730069 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
FE198_RS13540 2730066..2730359 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
FE198_RS13545 2730492..2733677 - 3186 WP_001550951.1 ribonuclease E -
FE198_RS13550 2734250..2735209 + 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(81-144)

Antitoxin

(14-88)


Sequences


Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T125673 WP_000613626.1 NZ_CP040390:c2730069-2729575 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T125673 NZ_CP040390:c2730069-2729575 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT125673 WP_001110447.1 NZ_CP040390:c2730359-2730066 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT125673 NZ_CP040390:c2730359-2730066 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0T0H9


Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References