Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 208326..208933 Replicon plasmid unnamed1
Accession NZ_CP040369
Organism Enterococcus faecium strain VB3240

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag FEF08_RS15475 Protein ID WP_002321032.1
Coordinates 208326..208676 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag FEF08_RS15480 Protein ID WP_002287514.1
Coordinates 208670..208933 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEF08_RS15440 203594..204328 + 735 WP_010729798.1 GntR family transcriptional regulator -
FEF08_RS15445 204312..205022 + 711 WP_010729797.1 GntR family transcriptional regulator -
FEF08_RS15450 205076..205756 + 681 WP_010861579.1 IS6-like element IS1216 family transposase -
FEF08_RS15455 205945..206529 - 585 WP_002299575.1 recombinase family protein -
FEF08_RS15460 206740..206943 - 204 WP_002299573.1 hypothetical protein -
FEF08_RS15465 206953..207375 - 423 WP_002288787.1 hypothetical protein -
FEF08_RS15470 207689..208302 + 614 Protein_229 recombinase family protein -
FEF08_RS15475 208326..208676 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FEF08_RS15480 208670..208933 - 264 WP_002287514.1 hypothetical protein Antitoxin
FEF08_RS15485 209274..209453 - 180 WP_002305761.1 hypothetical protein -
FEF08_RS15495 209834..210541 - 708 WP_002353391.1 hypothetical protein -
FEF08_RS15500 210534..210971 - 438 WP_002296237.1 hypothetical protein -
FEF08_RS15505 210986..211237 - 252 WP_002296238.1 hypothetical protein -
FEF08_RS15510 211237..211443 - 207 WP_002296239.1 hypothetical protein -
FEF08_RS15520 211884..212144 - 261 WP_002296240.1 hypothetical protein -
FEF08_RS15525 212510..212995 - 486 WP_002296241.1 single-stranded DNA-binding protein -
FEF08_RS15530 212998..213897 - 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(2'')-Ia / aac(6')-aph(2'') - 1..258646 258646


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T125653 WP_002321032.1 NZ_CP040369:c208676-208326 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T125653 NZ_CP040369:c208676-208326 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT125653 WP_002287514.1 NZ_CP040369:c208933-208670 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT125653 NZ_CP040369:c208933-208670 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References