Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3718984..3719620 Replicon chromosome
Accession NZ_CP040367
Organism Priestia flexa isolate 1-2-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A0V8JQ27
Locus tag FED53_RS19705 Protein ID WP_025910606.1
Coordinates 3718984..3719334 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1N6ZQI9
Locus tag FED53_RS19710 Protein ID WP_025910605.1
Coordinates 3719339..3719620 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FED53_RS19670 3714548..3715342 - 795 WP_025910613.1 RNA polymerase sigma factor SigB -
FED53_RS19675 3715308..3715793 - 486 WP_061785866.1 anti-sigma B factor RsbW -
FED53_RS19680 3715790..3716122 - 333 WP_025910611.1 anti-sigma factor antagonist -
FED53_RS19685 3716185..3717198 - 1014 WP_138117468.1 PP2C family protein-serine/threonine phosphatase -
FED53_RS19690 3717210..3717611 - 402 WP_025910609.1 anti-sigma regulatory factor -
FED53_RS19695 3717615..3717977 - 363 WP_035321022.1 STAS domain-containing protein -
FED53_RS19700 3717974..3718810 - 837 WP_138117469.1 STAS domain-containing protein -
FED53_RS19705 3718984..3719334 - 351 WP_025910606.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FED53_RS19710 3719339..3719620 - 282 WP_025910605.1 hypothetical protein Antitoxin
FED53_RS19715 3719800..3720972 - 1173 WP_138117470.1 alanine racemase -
FED53_RS19720 3721161..3722177 - 1017 WP_025910603.1 outer membrane lipoprotein carrier protein LolA -
FED53_RS19725 3722291..3722659 - 369 WP_025910601.1 holo-ACP synthase -
FED53_RS19730 3722760..3723344 + 585 WP_138117610.1 rhomboid family intramembrane serine protease -
FED53_RS19735 3723380..3724348 - 969 WP_138117471.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.94 Da        Isoelectric Point: 5.1442

>T125651 WP_025910606.1 NZ_CP040367:c3719334-3718984 [Priestia flexa]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDDALQISVGLIDF

Download         Length: 351 bp

>T125651 NZ_CP040367:c3719334-3718984 [Priestia flexa]
TTGGTAGTCAAACGCGGCGACGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGAGGCGTTCGCCCTGT
ACTTGTCATCCAAAATGATATTGGAAATCGGTTTAGTCCGACTGTCATTGTTGCTGCAATTACTGCTCAAATTCAAAAGG
CAAAGTTGCCTACGCATGTAGAAATTAATGCGAAAAAGTATGGGTTTGAGCGCGATTCAGTTATTTTGCTAGAGCAAATT
CGAACAATTGACAAACAGCGACTAACTGATAAAATCACCCATTTAGATGATGAAATGATGGACAGAGTGGATGATGCCTT
GCAAATCAGTGTAGGACTTATCGACTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10654.20 Da        Isoelectric Point: 9.5610

>AT125651 WP_025910605.1 NZ_CP040367:c3719620-3719339 [Priestia flexa]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSEFIYQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAES
EANHTVDRLVSGG

Download         Length: 282 bp

>AT125651 NZ_CP040367:c3719620-3719339 [Priestia flexa]
GTGTCTGAAGCGAGCGCAACTACAGAAATCCTGGTAAGATTGCCGAAAAATTTAGTATCGGAACTTGACCTTATGGTGAA
ACAAGAAAATGGTAACAGAAGCGAATTTATTTACCAAGCAACGAAATTATACCTTCGTGAGCGAAAAAAACGCCATATTC
GCGAGTCTATGCGTCGCGGATATATGGAGATGGCAAAGATAAATTTAAACATTGCTTCTGAAGCTTTTTTAGCTGAGTCT
GAAGCAAACCACACAGTGGATCGTTTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V8JQ27

Antitoxin

Source ID Structure
AlphaFold DB A0A1N6ZQI9

References