Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3551718..3552308 Replicon chromosome
Accession NZ_CP040363
Organism Klebsiella pneumoniae strain R50

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag FEM43_RS17620 Protein ID WP_022615588.1
Coordinates 3551976..3552308 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag FEM43_RS17615 Protein ID WP_117074187.1
Coordinates 3551718..3551975 (+) Length 86 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEM43_RS17585 3547544..3547786 - 243 WP_069376237.1 hypothetical protein -
FEM43_RS17590 3548053..3549801 + 1749 WP_102014240.1 hypothetical protein -
FEM43_RS17595 3549855..3550436 + 582 WP_099184326.1 hypothetical protein -
FEM43_RS17600 3550719..3551180 + 462 WP_004213450.1 hypothetical protein -
FEM43_RS17605 3551177..3551383 + 207 WP_020805021.1 helix-turn-helix domain-containing protein -
FEM43_RS17615 3551718..3551975 + 258 WP_117074187.1 antitoxin Antitoxin
FEM43_RS17620 3551976..3552308 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FEM43_RS17630 3552630..3554066 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
FEM43_RS17640 3554432..3555886 - 1455 WP_004148975.1 AMP nucleosidase -
FEM43_RS17645 3556016..3556261 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-108)

Antitoxin

(4-84)


Sequences


Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T125641 WP_022615588.1 NZ_CP040363:3551976-3552308 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T125641 NZ_CP040363:3551976-3552308 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9426.96 Da        Isoelectric Point: 4.4620

>AT125641 WP_117074187.1 NZ_CP040363:3551718-3551975 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCVPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT125641 NZ_CP040363:3551718-3551975 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGTTCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2


Antitoxin

Source ID Structure

References