Detailed information of TA system TA125638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1706648..1707273
• Replicon: chromosome
• Accession: NZ_CP040363
• Organism: Klebsiella pneumoniae strain R50

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FEM43_RS08630
• Protein ID: WP_142778387.1
• Coordinates: 1706648..1707031 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: FEM43_RS08635
• Protein ID: WP_004150355.1
• Coordinates: 1707031..1707273 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEM43_RS08615	1704013..1704915	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
FEM43_RS08620	1704912..1705547	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
FEM43_RS08625	1705544..1706473	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
FEM43_RS08630	1706648..1707031	-	384	WP_142778387.1	type II toxin-antitoxin system VapC family toxin	Toxin
FEM43_RS08635	1707031..1707273	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
FEM43_RS08640	1707478..1708395	+	918	WP_040215786.1	alpha/beta hydrolase	-
FEM43_RS08645	1708409..1709350	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
FEM43_RS08650	1709395..1709832	-	438	WP_117074358.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FEM43_RS08655	1709829..1710689	-	861	WP_004146232.1	virulence factor BrkB family protein	-
FEM43_RS08660	1710683..1711282	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14423.70 Da
• Isoelectric Point: 7.2773

>T125638 WP_142778387.1 NZ_CP040363:c1707031-1706648 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSRRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T125638 NZ_CP040363:c1707031-1706648 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCAGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATAACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT125638 WP_004150355.1 NZ_CP040363:c1707273-1707031 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT125638 NZ_CP040363:c1707273-1707031 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9