Detailed information of TA system TA125634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 147600..148270
• Replicon: plasmid p.P20Z
• Accession: NZ_CP040355
• Organism: Klebsiella pneumoniae strain P20

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: FEZ11_RS26225
• Protein ID: WP_004213072.1
• Coordinates: 147600..148043 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: FEZ11_RS26230
• Protein ID: WP_004213073.1
• Coordinates: 148040..148270 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEZ11_RS26190 (FEZ11_26320)	143009..143284	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
FEZ11_RS26195 (FEZ11_26325)	143347..143838	+	492	WP_011251327.1	DM13 domain-containing protein	-
FEZ11_RS26200 (FEZ11_26330)	143887..144807	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
FEZ11_RS26205	144898..145301	+	404	Protein_154	GAF domain-containing protein	-
FEZ11_RS26210	145819..146456	-	638	Protein_155	mucoid phenotype regulator RmpA2	-
FEZ11_RS26215 (FEZ11_26360)	146873..147177	+	305	Protein_156	transposase	-
FEZ11_RS26220	147200..147451	-	252	WP_186987481.1	hypothetical protein	-
FEZ11_RS26225 (FEZ11_26370)	147600..148043	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
FEZ11_RS26230 (FEZ11_26375)	148040..148270	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FEZ11_RS26235 (FEZ11_26380)	148878..150011	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
FEZ11_RS26240 (FEZ11_26385)	150027..150320	+	294	WP_004213076.1	hypothetical protein	-
FEZ11_RS26245 (FEZ11_26390)	150310..150516	-	207	WP_004213077.1	hypothetical protein	-
FEZ11_RS26250 (FEZ11_26395)	150868..151158	+	291	WP_004213078.1	hypothetical protein	-
FEZ11_RS26255 (FEZ11_26400)	151148..152047	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA	1..223144	223144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T125634 WP_004213072.1 NZ_CP040355:c148043-147600 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T125634 NZ_CP040355:c148043-147600 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT125634 WP_004213073.1 NZ_CP040355:c148270-148040 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT125634 NZ_CP040355:c148270-148040 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620