Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 42546..43456 Replicon plasmid p.P20Z
Accession NZ_CP040355
Organism Klebsiella pneumoniae strain P20

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag FEZ11_RS25680 Protein ID WP_004026354.1
Coordinates 42986..43456 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag FEZ11_RS25675 Protein ID WP_004026357.1
Coordinates 42546..42989 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ11_RS25645 (FEZ11_25765) 37564..38085 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
FEZ11_RS25650 (FEZ11_25770) 38082..39035 + 954 Protein_43 ferric citrate uptake sigma factor regulator FecR -
FEZ11_RS25655 (FEZ11_25775) 39122..41248 + 2127 WP_029884227.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
FEZ11_RS25660 (FEZ11_25780) 41323..41448 + 126 Protein_45 Fe3+-citrate ABC transporter substrate-binding protein -
FEZ11_RS25665 (FEZ11_25785) 41623..41844 + 222 WP_004213643.1 DUF2188 domain-containing protein -
FEZ11_RS25670 (FEZ11_25790) 41942..42445 + 504 Protein_47 DUF4113 domain-containing protein -
FEZ11_RS25675 (FEZ11_25795) 42546..42989 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
FEZ11_RS25680 (FEZ11_25800) 42986..43456 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
FEZ11_RS25685 (FEZ11_25805) 43911..44099 - 189 WP_223271245.1 hypothetical protein -
FEZ11_RS25690 (FEZ11_25810) 44713..44973 - 261 WP_004026352.1 hypothetical protein -
FEZ11_RS25695 (FEZ11_25815) 45278..46246 + 969 WP_004225014.1 IS5 family transposase -
FEZ11_RS25700 (FEZ11_25820) 46609..46908 - 300 Protein_53 IS481 family transposase -
FEZ11_RS25705 (FEZ11_25825) 46945..47642 + 698 WP_223175001.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA 1..223144 223144


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T125633 WP_004026354.1 NZ_CP040355:42986-43456 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T125633 NZ_CP040355:42986-43456 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT125633 WP_004026357.1 NZ_CP040355:42546-42989 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT125633 NZ_CP040355:42546-42989 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References