Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5168388..5169013 Replicon chromosome
Accession NZ_CP040354
Organism Klebsiella pneumoniae strain P20

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FEZ11_RS25070 Protein ID WP_002882817.1
Coordinates 5168388..5168771 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FEZ11_RS25075 Protein ID WP_004150355.1
Coordinates 5168771..5169013 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ11_RS25055 (5165754) 5165754..5166656 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FEZ11_RS25060 (5166653) 5166653..5167288 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FEZ11_RS25065 (5167285) 5167285..5168214 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FEZ11_RS25070 (5168388) 5168388..5168771 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FEZ11_RS25075 (5168771) 5168771..5169013 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FEZ11_RS25080 (5169218) 5169218..5170135 + 918 WP_029884108.1 alpha/beta hydrolase -
FEZ11_RS25085 (5170149) 5170149..5171090 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FEZ11_RS25090 (5171135) 5171135..5171572 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FEZ11_RS25095 (5171569) 5171569..5172429 - 861 WP_002882807.1 virulence factor BrkB family protein -
FEZ11_RS25100 (5172423) 5172423..5173022 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T125632 WP_002882817.1 NZ_CP040354:c5168771-5168388 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T125632 NZ_CP040354:c5168771-5168388 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCAGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT125632 WP_004150355.1 NZ_CP040354:c5169013-5168771 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT125632 NZ_CP040354:c5169013-5168771 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References