Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4555044..4555854 Replicon chromosome
Accession NZ_CP040354
Organism Klebsiella pneumoniae strain P20

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag FEZ11_RS22215 Protein ID WP_004178461.1
Coordinates 4555044..4555577 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A483I2M4
Locus tag FEZ11_RS22220 Protein ID WP_004222378.1
Coordinates 4555588..4555854 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEZ11_RS22210 (4553875) 4553875..4554996 + 1122 WP_002887282.1 cupin domain-containing protein -
FEZ11_RS22215 (4555044) 4555044..4555577 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
FEZ11_RS22220 (4555588) 4555588..4555854 - 267 WP_004222378.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
FEZ11_RS22225 (4555957) 4555957..4557390 - 1434 WP_004222374.1 Cu(+)/Ag(+) sensor histidine kinase -
FEZ11_RS22230 (4557380) 4557380..4558063 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
FEZ11_RS22235 (4558235) 4558235..4559620 + 1386 WP_004222371.1 efflux transporter outer membrane subunit -
FEZ11_RS22240 (4559638) 4559638..4559982 + 345 WP_004222368.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T125630 WP_004178461.1 NZ_CP040354:c4555577-4555044 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T125630 NZ_CP040354:c4555577-4555044 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9969.44 Da        Isoelectric Point: 10.1757

>AT125630 WP_004222378.1 NZ_CP040354:c4555854-4555588 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLSEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT125630 NZ_CP040354:c4555854-4555588 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAGTGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A483I2M4

References